| Ischemic cerebrovascular disease is cerebral infarction, which brings great harm to human health. There are various causes of cerebral infarction, causing nerve cells necrosis or apoptosis, neurological dysfunction aggravated, eventually lead to irreversible brain damage. Cerebral infarction can stimulate neurogenesis and let neural stem cells into the damaged areas, used to repair damaged nerve function and role. Therefore, promote nerve regeneration after stroke can help restore neurological function. According to research in recent years, Kangnaoye pretreatment have neuroprotective effect and promote angiogenesis after cerebral ischemia-reperfusion injury in rats, and its rarely reportede about nerve regeneration after cerebral ischemia reperfusion. Therefore, this experiment is to determined the role of Kangnaoye for nerve function and to observe the effect of the expression of growth associated protein-43(GAP-43), nerve growth factor(NGF), outgrowth inhibitor-A(Nogo-A) by making cerebral ischemia reperfusion model through the method of immunohistochemical, so as to do further research in the aspect of Kangnaoye pretreatment promoting nerve regeneration after cerebral ischemia reperfusion.150 healthy adult male Sprague Dawley rats were randomly divided into 5 groups: Kangnaoye high, middle and low dosages groups(24g·kg-1·d-1、12g·kg-1·d-1、6g·kg-1·d-1), sham operated group and model group. Longa method was used to establish the middle cerebral artery occlusion(MACO) model. TTC staining method was used to observe infarction volume change in rats after 24 hours. Collecting specimens in 1 d, 3 d, 7d and 14 d, HE staining of the cells was used to observe the morphology changes in cerebral infarction area. Immunohistochemical method was used to observe the change of GAP-43 after cerebral ischemic and the expression of NGF and Nogo-A protein. The nervous function deficit scores were evaluated at the 2 h, 1 d, 3 d, 7 d, 14 d after reperfusion.Neurological deficit scores of rats in model group were significantly higher then sham-operated group(P<0.01), and have larger infarct volume range.In tne model group, the number of neurons in cerebral ischemia was significantly reduced, scattered arrangement, broadening the gap between cells, nuclear condensation stain, the difference was statistically significant(P<0.01). Neurological function Kangnaoye pretreatment was significantly higher than the model group(P<0.05). Compared with the ischemic area of model group, Kangnaoye pretreatment had a high erexpression of GAP-43 and NGF in ischemia-reperfusion area, at the same time the expression of NOGO-A were lower, reduce infarct volume and morphological changes, which has significant differences(P<0.05 or P<0.01).In summary, Kangnaoye can promote nerve regeneration in rats, recovery of motor function in rats with cerebral infarction, reduce the infarction area. The possible mechanism may be through the promotion of GAP-43 and NGF expression in the surrounding area of infarction, and may inhibit NOGO-A expression, then promote the repair of damaged neurons, enhancing axon regeneration as well as synaptic reconstruction. We think that Kangnaoye may promot nerve regeneration after cerebral ischemia reperfusion. |
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