The Establishment Of The Specimen Bank Of The Core Family Of Liver Cancer And The Investigation And Analysis Of The Compatriots Of The Proband

PART 1: THE ESTABLISHMENT OF LIVER CANCER CORE FAMILY SPECIMEN DATABASEObjective:Established hepatoma nuclear family sample storehouse,for genetic susceptibility to primary hepatocellular carcinoma and family aggregation of studies provide blood samples and clinical data;on hepatoma nuclear family of investigation,detection and analysis of the study of hepatitis B virus infection;prevention publicity and cancer screening for hepatocellular carcinoma(HCC)core family members;to carry on the discussion to the problems existing in hepatoma nuclear family sample library of.Methods:Hepatoma nuclear families of probands were patients to the hospital medical record room query has been discharged,or to the ward to query in the hospital records of patients;medical units data or community and village liver cancer screening data query,engagement in hepatoma nuclear family of objects to meet the,understand the incidence,HBV infection history,tumor family history,home address,contact information,inform the significance of the study,get their understanding and cooperation,and HCC proband parents HCC etiology,diagnosis,treatment and prevention knowledge propaganda.In the course of the establishment of liver cancer core family,the field epidemiology and experimental epidemic methods were adopted..Hepatocellular carcinoma(HCC)core Department of field epidemiological investigation,the contents of the questionnaire include:demographic data,past disease history,living habits,hepatitis B surface antigen positive status,long-term oral medication history,family history of malignant tumor and.HCC core pedigrees were blood samples were collected:the consent of liver cancer probands and their parents' consent,the mobilization of the research object to my hospital for hepatitis B two half and half,alpha fetal protein(AFP),liver function test and liver B ultra inspection for some inconvenience to the hospital,sent door-to-door survey of all subjects were collected fasting peripheral venous blood 8ml,of which 3 ml loaded into the blank of the vacuum blood collection,and the non anticoagulant and separating serum hepatitis B two half and half,alpha fetoprotein(AFP)and liver function(mainly-to alanine transferase,aspartic acid transferase)and other biochemical index detection;the remaining 5 ml of blood,the specimens are loaded with anticoagulant EDTA vacuum blood sampling.The blood samples back to the lab,anti coagulation of blood samples after centrifugation the stratification of plasma and white blood cells,red blood cells respectively into 1.5ml frozen depository,numbered in a unified way and stored in the freezer at-20 DEG C,for the next step to do liver cancer genetics and gene analysis to do a full preparation.Results:A total of 251 cases of liver cancer core family,with complete blood samples and epidemiological data,for HCC genetic susceptibility and familial aggregation of research foundation.In this study,251 liver cancer probands were HBsAg positive rate 92.03%231/251,once again confirmed the HB V infection and HCC has a close relationship:in 251 cases of hepatocellular carcinoma(HCC)core pedigrees have family history of liver cancer pedigrees in 30 cases,accounting for the total number of 11.95%(30/251),including 15 cases of the proband's level relatives(the proband's parents and brothers and sisters);through the detection of hepatitis B two half and half,AFP and B ultra inspection of parents of proband screening,and the new discovery 1 cases of male patients with hepatocellular carcinoma and postoperative pathology showed liver cell carcinoma.Conclusion:(1)HCC core pedigree specimen database establishment is a pioneering work,through the establishment of the hepatoma nuclear family sample database,make full use of the resources in this region,provide experimental specimens and basic data for further study of genetic susceptibility to primary hepatocellular carcinoma,provide more convincing evidence for revealing the mechanism of genetic susceptibility to primary hepatocellular carcinoma and filtering.Laboratory testing(2)of hepatocellular carcinoma(HCC)core family members suggest,liver cancer probands,mother of the HBV infection rate than HCC proband father high infection rate of HBV,which may has certain relationship with HBV vertical transmission.(3)of hepatocellular carcinoma(HCC)in the proband's relatives are high-risk population of hepatocellular carcinoma,in the establishment process of hepatocellular carcinoma(HCC)core pedigrees,we through screening for hepatocellular carcinoma(HCC)core family members,the newly discovered HCC patients(n= 1),postoperative cases of hepatocellular carcinoma(HCC).Therefore,the screening of the relatives of the liver cancer first card can improve the early diagnosis rate and survival rate of HCC.(4)in hepatoma nuclear family sample library establishment process in the research object heart,social ethics,sample storehouse management problems discussion,accumulated experience and cultivate talents,in order to provide reference to build the development of other subjects and larger specimens libraryPART 2:THE INVESTIGATION AND ANALYSIS OF THE SPECIMEN STOREHOUSE OF NUCLEARFAMILY SAMPLE BANK OF PLCOjective:251 cases of liver cancer core family first card compatriots forinvestigation and analysis.Methods:Fellow probands of 251 cases of hepatocellular carcinoma(HCC)core family contact,inform the purpose and significance of the research,request of the subject and objects of investigation benefit and obtain the understanding and cooperation research.Research points for the field epidemiology and experimental epidemiological method,to a new copy number variation of liver cancer probands are lineal relatives are investigated.The contents of the questionnaire include:demographic data,past disease history,living habits,hepatitis B surface antigen positive status,long-term oral medication history,family history of malignant tumor and.For all of the subjects were morning fasting venous blood 8ml,which is then 3ml loaded into the blank of the vacuum blood collection,and HBV DNA of non anticoagulative serum separation,alpha fetal protein and hepatic function(mainly alanine aminotransferase(ALT),aspartic acid transferase)of detection index;the remaining 5ml blood specimens are loaded with EDTA anticoagulant vacuum blood collection.The blood samples back to a laboratory after,the anticoagulant blood samples from centrifugal 15min and after centrifugation the layered plasma,white blood cells and red blood cells were into 1.5ml frozen depository,numbered in a unified way and stored in the freezer at-20 DEG C,in order to verify the liver cancer germline copy number variation provided blood samples and epidemiological data?Results:The final follow-up of the 98 cases of liver cancer core family of the first card of the compatriots.98 families of the original population of 686 people,including the first card 98 people,196 parents,392 compatriots,family average population of 7 people(range 4-12 people),including 399 men,287 women.There are 24 cases of liver cancer first card Shang Cunhuo,accounting for 24.49%(24/98).There are 6 cases of HCC proband father died of liver cancer,5 cases of liver cancer probands,mother died of liver cancer,with 31 cases of liver cancer probands were brothers and sisters death.Among them,29 cases died of liver cancer,1 case died of lymphoma,1 case died of AIDS.The HCC proband siblings of the survey results,a total of 167 proband sibling,the first card brothers,109 and probands and sisters 58 people;investigation team found that contains two cases of patients with hepatocellular carcinoma pedigrees with 27.Hepatoma nuclear family proband sibling laboratory test results are as follows.167 cancer probands with the number of siblings of HBsAg positive 65 people,the positive rate for 38.92%65/167.The positive rate of HBsAg in liver nuclear family proband siblings were significantly higher than that of HBsAg positive rate of natural population in this regionConclusion:The final follow-up of the 98 cases of liver cancer core family of the first card of the compatriots.98 families of the original population of 686 people,including the first card 98 people,196 parents,392 compatriots,family average population of 7 people(range 4-12 people),including 399 men,287 women.There are 24 cases of liver cancer first card Shang Cunhuo,accounting for 24.49%(24/98).There are 6 cases of HCC proband father died of liver cancer,5 cases of liver cancer probands,mother died of liver cancer,with 31 cases of liver cancer probands were brothers and sisters death.Among them,29 cases died of liver cancer,1 case died of lymphoma,1 case died of AIDS.The HCC proband siblings of the survey results,a total of 167 proband sibling,the first card brothers,109 and probands and sisters 58 people;investigation team found that contains two cases of patients with hepatocellular carcinoma pedigrees with 27.Hepatoma nuclear family proband sibling laboratory test results are as follows.167 cancer probands with the number of siblings of HBsAg positive 65 people,the positive rate for 38.92%65/167.The positive rate of HBsAg in liver nuclear family proband siblings were significantly higher than that of HBsAg positive rate of natural population in this region.