Study On The Effect Of Acupuncture At Selected Points Along The Meridian On The MT Content Of The Pineal Gland And The Expression Of SCN MT₁ And MT₂ MRNA In Insomnia Rats

Objective:To observe the effects of the acupoints of different meridians on the Melatonin?MT?content of the pineal gland and the expression of Melatonin receptor1?MT₁?and Melatonin receptor 2?MT₂?in the rats,and to explore the effect of the selected acupoints on the distribution of acupoints.Methods:60 SD rats were randomly divided into normal control,model,same meridian:“Bahui”?GV 20?-“Shenmen”?HT 07,3-acupoints?,related meridian:“Bahui”?GV 20?-“Sanyinjiao”?SP 06,3-acupoints?,non-acupoint:“Bahui”?GV 20?-non-acupoint?3-acupoints?groups,with 12 rats in each group.Insomnia model was eatablished by Intraperitoneal injection of para chlorophenylala-nine suspension.Acupuncture therapy once daily for 7 days.Observation rat circadian activity,sleep time,sleep latency,using immune histochemical method to detect the pineal gland of rats MT of grey value,using real-time fluorescent quantitative PCR method to detect the suprachiasmatic nucleus in rats MT₁ and MT₂ mRNA expression.Results:1?After modeling,pentobarbital righting the experimental results show that the model group the sleep latency than before modeling extension?P<0.05?,sleep duration was shortened before modeling?P<0.05?,and to compare the difference with the control group?P<0.05?,suggesting that the model was successful.Compared with normal group,the sleep latency period of sodium pentobarbital sleep was increased in the model group?P<0.05?,and that of sleep duration.Compared with model group,the sleep latency period was decreased in the same meridian group,related meridian group,non-acupoint group?P<0.05?,and the sleep duration was increased in the same meridian group?P<0.05?.2?Diet:compared with the blank group,the model group to reduce consumption,the difference was statistically significant?P<0.05?;compared with the model group,Baihui plus Shenmen group increase food intake?P<0.05?;comparison between treatment groups,Baihui plus Shenmen group than Baihui and Sanyinjiao group,Baihui and non acupoint group?P<0.05?.The amount of drinking water:model group was less than that of control group?P<0.05?;Baihui plus Shenmen group,Baihui and Sanyinjiao group,Baihui and non acupoint group less than the model group?P<0.05?;the treatment group were no difference?P>0.05?.3?The body weight increase of the rats compared with the control group,model group,body weight growth quantity is very few,the difference was statistically significant?P<0.05?;compared with the model group,Baihui plus Shenmen group,Baihui and Sanyinjiao group weight increase more?P<0.05?;comparison between treatment groups,Baihui plus Shenmen group than Baihui and Sanyinjiao Baihui plus group,non acupoint group?P<0.05?.4?Comparison of the gray value of pineal body:compared with the blank group,MT gray value of the model group was increased;Compared with the model group,the MT gray value of Baihui plus Shenmen group,Baihui plus Sanyinjiao group was significantly reduced?P<0.05?.There was no significant difference in MT gray value between the three groups in each treatment group?P?0.05?,but there was a tendency in the Baihui plus Shenmen group to be lower than the other two groups.5?The mRNA content of MT₁ and MT₂ was compared between the two groups:compared with the blank group,the mRNA expression of MT₁ and MT₂ in the model group was significantly reduced?P<0.01?.After acupuncture,compared with the model group,the expression level of MT₁ and MT₂ mRNA in the Baihui plus Shenmen group,Baihui plus Sanyinjiao group and Bahui plus non-acupoint group was significantly increased?P<0.01?.The expression of MT₁ mRNA was significantly higher than that of Bahui plus non-acupoint group?P<0.01?,and the mRNA expression level of MT₂ was significantly higher than that of the other two groups?P<0.01?.Conclusion:Acupuncture treatment can increase the MT content of conarium and the expression of MT₁ and MT₂ mRNA in the suprachiasmatic nucleus of insomnia rats.Fixed local acupoints combined with circulation point selection can improve the compatibility of acupoints.