The Inhibitory Effect And Mechanism Of Caffeine In Gastric Cancer Cells

Caffeine,one of the most widely consumed substances derived from beverages,has anti-cancer effects in several types of cancer.This study aims to examine the anti-cancer effects of caffeine on gastric cancer(GC)cells(MGC-803 and SGC-7901)in vitro and to determine whether the apoptosis-related Caspase-9/3 pathway is associated with these effects.The sustained anti-proliferative effects of caffeine on gastric cancer were also investigated.GC cell viability and proliferation were evaluated by harvesting and counting cells at preset time points after treatment with specific concentrations of caffeine.Flow cytometry was performed to assess cell cycle dynamics and apoptosis.Western blot analysis was conducted to detect the activity of the above signalling pathway.Our data show that caffeine treatment significantly suppressed GC cell growth and viability and induced apoptosis by enhancing the Caspase-9/3 pathway.Moreover,the anti-cancer effects of caffeine seemed to be sustained,as the Caspase-9/3 pathway remained active after caffeine withdrawal.In conclusion,caffeine functions as a sustained anti-cancer agent by activating the Caspase-9/3 pathway,which indicates that it may play a therapeutic role in gastric cancer.