Comparison Of Ingredients Before And After "sweat" And Research On Quality Control

bjectives1.Sweating for radix dipsaci influence of total saponins,water-soluble extract and alcohol soluble extract.2.To set up the characteristic fingerprinting of cruded and sweated Radix Dipsaci.3.Simultaneous determination of eight ingredients in cruded and sweated Radix Dipsaci.4.To establish the rapid identification model of cruded and sweated Radix Dipsaci,and rapid determination method of the contents of eight ingredients based on NIR.5.To develop a HPLC method for simultaneous determination of loganin and Asperosaponin VI in rat serum and study the pharmacokinetics of loganin and Asperosaponin VI in cruded and sweated Radix Dipsaci.Methods1.By using UV chromogenic method and hot dipping method investigate sweating for radix dipsaci influence of total saponins,water-soluble extract and alcohol soluble extract.2.Choose cruded and sweated Radix Dipsaci in 20 batches respectively,and carry out gradient elution in acetonitrile-0.05%phosphoric acid,with flow velocity 1.0mL.min-1,column temperature 25?,recording duration 120 min temperature 30?.Then set up the characteristic fingerprinting of cruded and sweated Radix Dipsaci by using Evaluation System of the Similarity of TCM Chromatographic fingerprinting Version 2.0 made by China Pharmacopoeia Commission.3.The content of eight main components in cruded and sweated Radix Dipsaci were determined by HPLC.4.In 213 batches of cruded samples and 234 batches of sweated samples processing from different producing areas,collect their NIR and establish qualitative identification model by discrimanant analysis method;determine the content of eight ingredients through HPLC method,establish the multiplicative correction model between NIR and estimated value through PLS,and predict the amount of eight ingredients in cruded and sweated Radix Dipsaci.5.Using HPLC-MS to determine pharmacokinetic parameters of loganin and Asperosaponin? in cruded and sweated Radix Dipsaci and drug-time curve in rat.Results1.After sweating,the contents of total saponins,water soluble extract and alcohol soluble extract of Radix dipsaci are all decreased.These results may be the caused by digestion of the related material in the sack covering dumps of sweating process.2.In the same retention duration,their chromatographic peak height,comparing the HPLC elution profile of cruded Radix Dipsaci have some changes to different extent.Among which in the elution profile of sweated Radix Dipsaci,the height of chromatographic peak is low,even to zero,while some new chromatographic peaks emerge.There are 35 common chromatographic peaks in the fingerprinting of cruded and sweated Radix Dipsaci,among which No.7,No.8,No.9,No.10,No.18,No.19,No.21 and No.29,are loganic acid,chlorogenic acid,caffeic acid,loganin,Isochlorogenic acid B,Isochlorogenic acid A,Isochlorogenic acid and Asperosaponin VI respectively.3.A method for simultaneous determination of eight active components including loganic acid,chlorogenic acid,caffeic acid,loganin,Isochlorogenic acid B,Isochlorogenic acid A,Isochlorogenic acid and Asperosaponin VI and it proves to be safe and stable.4.The identification model was developed by choosing cruded Radix Dipsaci spectrum for 7,756.86?4,006.00 cm-land sweated Radix Dipsaci spectrum for 8,562.39?4,528.04 cm-1."MSC+spectrum+Ns”was chose to the original spectral preprocessing,and then was verified by prediction set,identify with 100%accuracy.5.Maxium blood concentration of loganin and Asperosaponin VI and AUC is higher in cruded Radix Dipsaci.Conclusion1.Sweating for radix dipsaci reduced the content of total saponins,water-soluble extract and alcohol soluble extract.2.The established fingerprinting of cruded and sweated Radix Dipsaci can control the quality of these two kinds of samples.3.Eight main components in crude and sweated Radix Dipsaci have been determined simultaneously and a material foundation has been laid for further research on revealing the processing mechanism.4.The established NIR identification model can be used to identify crude and sweated Radix Dipsaci rapidly and accurately.The model has certain predict ability to prediction set.Therefore,test eight main components in crude and sweated Radix Dipsaci rapidly quickly based on NIR technology is feasible.5.Compared pharmacokinetic characteristics of loganin and Asperosaponin ? after the rat gastric drug delivery.It discovery that pharmacokinetic parameters,such as t1/2,MRT in cruded Radix Dipsaci is superior to sweated,which demonstrate cruded Radix dipsaci is easily absorbed in the body.