The Inhibitory Effect Of Somatostatin Analogs On Liver Cancer Cells And The Effect On CDk5 Expression

Objective Different concentration of growth inhibition change analogues (octretide peptides)add to the People HepG2liver cancer cell lines cultured in vitro.Observe the shape of the cells in the liver cancer cells to grow and liver cancer in change.Detect each group OD value In different time points Observe liver cancer cells of the value of the situation. Detect cycle grain dependence protein kinase5(CDK5) in the liver cancer cells of the expression by immunocytochemical method.Discuss growth inhibition change and their analogues to human liver cells inhibitory and to cycle grain dependence protein kinase5expression effectMethods1.Cultivate human liver cell lines (HepG2) in vitro. It was made into single-celled levitation liquid by pancreatic enzymes when the cells were paved with the bottle of70%-80%. Subculture as1:3scale.2. The experiment was divided into4groups as the concentration of peptide octretide. Control group(A) don't add octreotide.Experimental group:The concentration of peptide octretide is (B)0.002mg/ml,(C)0.006mg/ml,(D)0.010mg/ml.3.Observe liver cancer cells change shape and the growth of cells of different concentration groups under the microscope in the cart before the horse after drug acted48h.4.Watch The influence of different concentration of octretide peptides to liver cancer cell proliferation along with the extension of action time.5.Let cells grow on the sheet glass Detect the expression of CDK5with immunocytochemical methodResult1.Forms of cancer cells of experimental group had changed, lots of anchorage-dependent cell turned into bright state of circular from arborization Under the microscope in the cart before the horse2.OD decreased along with the increase of drug concentration at the same time. OD changed in accordance with gradient along with the extension of action time, the numerical value decreased gradually.It demonstrated that the proliferation of growth inhibition of Octreotide to HepG2is time dose dependent relationship, statistical significance The difference between any two groups have statistical significance.3.Express positive things of CDK5were Tan particles.Cytoplasm and caryon was filled with them.Dyeing degree changed in accordance with gradient. The larger octretide peptide drug concentrationwas,the lighter dyeing degree was.Caryon was sepia after sappanwood grain after dyeing.Cytoplasm was lighter relatively.The nucleus of the proportion of the light dye scaled-up along with increasing drug concentration.Conclusion Octreotide may restrain the growth of liver cancer cells.Down regulated the expression of CDK5of hepatocarcinoma.