Function Study Of HsfA1 And HsfBs Genes In Spinach

Spinach?Spinacia oleracea L.?is a cold-tolerant vegetable with a suitable temperature for vegetative growth of 15-20?,Over 25 C,the growth was poor,the yield was low,the quality was poor,and even died.When plants are subjected to heat stress,they produce a series of reactions from cells to physiological levels.This series of reactions is regulated by multiple genes.Among these genes,heat shock transcription factors are the major regulators,and they constantly activate and signal and amplify signals in stress responses,regulating the expression of downstream genes.Through genome-wide search and analysis,16 full-length genes of SoHsfs family were identified in spinach,There are 8 heat shock transcription factors of class A,6 heat shock transcription factors of class B,and 1 heat shock transcription factor of class C,and the full length of the coding region of each gene was cloned,and SoHsfs protein was predicted.Biological function.The specific anti-reverse function of SoHsfs gene has not been reported.Real-time quantitative RT-PCR analysis showed that SoHsfB1?SoHsfB2.1?SoHsfB2.2 and SoHsfA1 genes were expressed in all tissues of spinach.SoHsfB1?SoHsfB2.1?SoHsfB2.2 and SoHsfA1 genes increased with the occurrence of high temperature stress.SoHsfB1?SoHsfB2.1?SoHsfB2.2,as B heat shock transcription factors,had a positive response to heat stress.the recombinant yeast INVScl?pYES2?system was subjected to abiotic stresses such as salt,alkali,heavy metals,oxidation and drought.It was found that the genes of SoHsfB1?SoHsfB2.1?SoHsfB2.2 and SoHsfA1 could improve the ability of recombinant yeast to resist abiotic stresses in varying degrees.SoHsfB1 gene not only has the strongest salt tolerance and alkali tolerance?Na₂CO₃?,but also has the strongest resistance to oxidative stress and heavy metal?CuSO₄?;SoHsfA1 has the strongest alkali tolerance?NaHCO₃?and drought tolerance;SoHsfB2.1?SoHsfB2.2 gene has the strongest resistance to heavy metal?CdCl₂?.The cis-acting elements of SoHsfB1?SoHsfB2.1?SoHsfB2.2 and SoHsfA1genes were screened by yeast single hybridization.Through the functional analysis of stress resistance?high temperature,salt,alkali,heavy metal and drought stress?of Arabidopsis thaliana overexpressing SoHsfB1?SoHsfB2.1?SoHsfB2.2 and SoHsfA1genes,it was found that overexpressing SoHsfB1?SoHsfB2.1?SoHsfB2.2 and SoHsfA1genes participated in the response to abiotic stress during the germination of transgenic Arabidopsis,and improved the tolerance to abiotic stress during the germination of transgenic Arabidopsis thaliana.And the heat tolerance of transgenic Arabidopsis seedlings.This study laid a foundation for further in-depth study on the heat-tolerant mechanism of spinach,and provided theoretical support for the breeding of heat-tolerant varieties of spinach.