Identification Of Rice Heat-shock Proteins And Analysis On The Role Of Hsp70s In Response To Abiotic Stress

Heat shock proteins (Hsps) have been known as a class of proteins that perform afundamental role in protecting plants against abiotic stresses, such as drought, salinity,extremely low and high temperatures. However, in rice, except for small Hsps andHsp100family, rare Hsps have been reported and little consideration has been givento the role of Hsps in response to abiotic stress. Fortunately, the growing number ofbiological data make it possible to address these questions.Based on proteomic and gene chips data, twenty-seven heat shock proteins werescreened from rice proteome, including3clpB/Hsp100,3Hsp90,6Hsp70,3Hsp60and12sHsp family members in this paper.430Hsp70s interactors, which geneexpressions appeared to be strongly correlated with Hsp70s under abiotic stress, wereidentified. Furthermore,13interacting domains and28target motifs were presented inthese Hsp70s interactors by analyzing the structure feature of Hsp70s interactors.Finally, through gene ontology (GO) analysis, we found24GO terms of biologicalprocesses and5GO terms of molecular functions were enriched among the positivecorrelated interactors of Hsp70s. Meanwhile,23GO terms of biological processes and16GO terms of molecular functions were overrepresented among the negativecorrelated interactors of Hsp70s. The research endeavored to identify Hsps in rice andreveal the feature of rice Hsp70s in response to abiotic stresses.Moreover, we constructed a Protein-Protein Interaction Predictor (PPIP) and aRice Gene Expression Profile datebase for expermental biologists. These tools couldbe available at http://bioinformatics.fafu.edu.cn/yfwang/PPIP/ppip.php andhttp://bioinformatics.fafu.edu.cn/yfwang/array/index.php respectively.