Fluorescence Presentation And Digital Analysis Of Internal Information In Complex Environmental Solutions

Complex chemical system(CCS)is one of the most common and essential matter states in the nature.The research fields involve food,medicine,environment,agriculture,biology,and so on.One CCS usually consists of many chemical species,attending enormous linear and nonlinear coupling reactions.The complicated relationships among these species are extremely different from that in a simple or designed mixture.In this mixture,a large number of interactions among constituents are the essential sources of the external functions and effects.Although such cognition and consequent arms will bring a new prosperity to many natural sciences,e.g.chemistry,materials,biology,geology and environment,scientific community is at a losing of regarding the implementation of such researches presently.For overcoming the disadvantages in the ordinary pathways,in recent years,some researchers began to explore CCS.With the inspiration of BD and high-throughput experimentation(HTE),new methods or tools are expected to be created to express the vast internal relations in CCS.In the near 3 years,our team created and built a new strategy to express the internal relationships of CCS.The details are as follows.In a balanced CCS,when a chemical is added,the original equilibrium state would be perturbed more or less.Then the system shifted into another equilibrium state and displayed some specific results.In other words,the process can be described as "one perturbation to one expression(PtoE)".Similarly,when various independent perturbations input the CCS,individually,numerous PtoE process will provide a group of corresponding results.These various results are derived from the added chemical perturbations to the numerous relationships in the CCS.To make PtoEs more efficient and convenient in operation,it is necessary to design and combine numerous perturbation actions and result expressions into a structured form.By means of HTE,the perturbation actions can be combined on a flat surface.For result expression,we are apt to creating or improving some fluorescence imaging(FI)methods to visualize the features of CCS.In the obtained image,the difference of each point was related to a certain and known perturbation action.The experiments and results as follow:(1)Construction of L-chip.A proper chemical perturbance system was selected and structured by high-throughput combination method,which would provide a large number of parallel and distinguishable perturbance reactions.In order to obtain this perturbation system conveniently,our team distributed quantitatively the disturbance agents on a plane carrier by adopting the CMYK color model ink-jet printing.To begin with,a printing template,which could be named as L-template,was design delicately in the special drawing software.In this template,the reaction zone of perturbation system was a circle area which was made of two layers,and each layer consisted of three primary colors(cyan,magenta and yellow).Secondly,by positioning overprint,six disturbance agents would spray on the plane in the light of the L-template.Finally,an entirely new chemical composition plane named as L-chip was obtained.(2)Image Capture.In an ultrasonic atomizer,the samples were efficiently dispersed into vast micro-droplets,and a quantitative dispersing device was built to realize their uniform quantitative deposition into reactors,The testing showed that the average diameter of droplets was about 3.5?m and its equivalent volume was approximately 0.180pL after ultrasonic atomization.The expression of the PtoE was fulfilled by an imaging system(fluorescence imaging).Here,a series of fluorescence images obtained.(3)L-analysis.The information in L-images was considered to deposit in at least 3 aspects.The first is the visual pattern and color in L-images.The L-image for a certain sample is unique and characteristic while some L-images have some common features due to the similar species in samples.The second aspect is founded on the base,the PtbS of L-chip being known completely and located exactly.The internal chemical properties in a CCS are apt to be explored by virtue of the couple of L-image and the rules in the templated PtbS.The third is produced from the relationships of L-images from different samples,and these L-image relationships will provide matters an interlinking web.There are still a mass of job of building appropriate technology to exploit and use the information in L-images efficiently.(4)In addition,CCSs exist in many fields,and we chose several typical samples to attempt to establish and implement the proposed strategy.Those tested samples included some environment samples,humic matters.