Apigenin Based On Metabolomics Digestion And Absorption Mechanism And Anti-oxidative Stress Ability Research

The content of apiin is the highest in celery.At present,the research on apiin was focused on the separation,extraction and functional research.The study on the digestion and absorption of apiin had not been reported.The traditional methods of active research have failed to combine with the physiological environment,and flavonoids would be transformed or metabolized in the physiological environments.To overcome the deficiencies,the in vitro digestion model,Caco-2 monolayer model and HUVEC-C oxidative stress model were used to study the changes of content and antioxidant of apiin in different stages.The metabolites of polyphenols was explored with the non-target metabolomics technology in the intestinal absorption and exerting antioxidant.The study have provided a theoretical basis for further understanding of apiin in vivo digestion metabolic and active ingredients in celery.The results were as follows:(1)In vitro gastrointestinal digestion model was established to investigate the effect of concentration and digestion time on the digestion of apiin in the gastrointestinal physiology environment.HUVEC-C oxidative stress model was established to study the changes in the ability of antioxidative stress in the digestion process.The results showed that gastrointestinal fluid had little effect on the stability and the antioxidative stress ability of low concentration apiin(0.5 mg/mL).But the medium and high concentration of apiin had a significant change in gastrointestinal fluid(p<0.05),and showed a trend of decreasing first and then increasing.The results showed that gastrointestinal juice could promote the degradation or transformation of apiin;and the change of the antioxidative stress ability of apiin was positively correlated with its content.(2)The Caco-2 monolayer model and HUVEC-C oxidative stress model was established to study the changes of content and antioxidant activity of apiin during absorption.The metabolites was studyed by non-target metabolomics techniques during the absorption of apiin.The results showed that apiin was transported by passive transport and paracellular pathway.The polyphenols metabolites included 9 substance:6-gingerol,quercetin,apigenin-7-O-glucoside,quercetin,peonidin glucoside,cyanidin-3-0-2’-O-β-D-xylopyranosyl-β-D-glucopyranoside,New Zealand vitexin Ⅱ,rutin,isorhamnetin-3-Glucoside-4’-glucoside.By studying the changes of 9 polyphenols content in the transport fluid at different periods,it was found that except for 6-gingerol and quercetin,the other 7 polyphenols transported volumes showed a trend of increasing first and then decreasing,it’s due to that with the increased of time,the trans-shipment volume increased,the concentration difference between Caco-2 cells sides,the amount of transported substances gradually decreased.(3)HUVEC-C oxidative stress model was established to study the antioxidantive stress ability of apiin,and the metabolites was investigated by non-target metabolomics technology.The results showed that 0.1 mg/mL apiin could repair HUVEC-C oxidative stress cells.Compared with the blank group,the NO content increased by 0.84 times.The major polyphenols metabolites of apiin when it exerts anti-oxidative stress included five substances:catechin,rutin,trans-5-O-caffeoylquinic acid,soybean glucan,malva pigment 3-galactoside and apigenin.In summary,the gastrointestinal tract juice could promote the degradation or transformation of apiin.When apiin was absorbed by the Caco-2,it may be converted to gallophenol catechin,quercetin,apigenin-7-O-glucoside,quercitrin,peonidin glucose glycosides,cyanidin-3-O-2’-O-(3-D-xylopyranosyl-(3-D-glucopyranoside,New Zealand vitexin II,rutin,isorhamnetin-3-glucoside-4’-glucoside.Apiin was transported in vivo by passive transport and paracellular pathway,the P-gp and MRP would reduce its absorption.During the process of digestion and absorption,the ability of antioxidative stress of apiin was continue to change.After digestion and absorption,antioxidative stress ability was lower than not digested group.Apiin may be converted into catechin,rutin,trans-5-O-caffeoylquinic acid,soybean glucan,malva pigment 3-galactoside and apigenin.