The Microenvironment Offluid Shear Force Based Onthe Spoke-type Idt Electrodearray By

In recent years,the technology of microfluidic chip is developing in the field of biological medicine,etc.,especially in the area of cell culture in vitro.Perfusion system is often used in the biochips to complete the pump of high conductivity fluids such as cell culture medium in cell culture.Comb-type parallel interdigital electrode arrays are used in conventional infusion system as a drive unit.Although the array structure can realize high throughput and low consumption of fluid pumping,it did not consider the problem that the flow shear stress micro-environment of cells in the system is instable or does not meet the changing damands of growing,causing the decreasing rate of cell viability.Thus,this topic has designed a new type of structure of perfusion culture chip to make up for the deficiency,which can create a variety of flow shear stress micro-environments for cells,and cells can be chosen according to their own needs.This article is based on the development status of AC electric parallel electrodes of electrothermal micro pump,and optimized the parameter of the the electrode array size and AC electric field on the basis of rectification of alternating electric field,identifies the structure of spoke-type IDT electrode array patterns and finally realizes the pump of biofluid circulation of low voltage AC for cell culture medium.The high conductivity fluid in the chips has Joule heating effect in the non-uniform electric field,and the pump is delivered under the action of electrothermal force.Due to the particularity of the electrode structure,the chip can provide the cell with the fluid shear force environment that can meet its growth needs while circulating the fluid.In this paper,the feasibility of the chip is verified through theoretical research,numerical simulation analysis and experiment.It conducts theoretical Analysis on each influence factor of force on the internal fluid through chips,uses finite element simulation analysis software to establish a three-dimensional model to simulate the pumping process of fully coupling simulation,compares design model with the electric field,flow field and temperature field of ordinary parallel micro pump model to prove that the chips in the subjects are rich in the environment.With the parameterized scanning of the key parameters of the scanning electrode in strong coupling models,the optimal parameters are concluded and the relationship between net flow velocity and Ac voltage is explored.According to theelectrode parameters based on the simulation optimization,the experiment micro pump chips are processed and system platform is formed.We used NaCl-polystyrene microspheres suspension test similar to electrical conductivity and cell culture medium as experimental microfluidics.It also observes the multi flow shear stress micro-environment of static microfluidic chip micro pump pumping effect under the action of different voltage.The experimental results show that the chip can create a multi-fluid shear force microenvironment in the process of pumping fluid,the flow of the fluid is in the form of“V”,and the effect is optimized with the increase of voltage value.