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Research On Metal Nanowire Grating SPR Biosensor

Posted on:2021-02-06Degree:MasterType:Thesis
Country:ChinaCandidate:S Y ZuoFull Text:PDF
GTID:2428330611472116Subject:Detection Technology and Automation
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Surface plasmon resonance is a sensitive surface analysis technology that detects the change in dielectric constant caused by molecular adsorption on heavy metal films.Since this method has the advantages of fast,real-time,and in situ detection of biomolecules,it has been widely used in the study of biomolecule interactions since the 1990 s.The sensor chip is the core component in the SPR detection sensor.Used to capture biomolecules that are coupled to sensors and can react specifically with them.Therefore,the research on sensor chips usually becomes the research focus of this detection technology.Gold nanomaterials have become popular choices for biosensing media due to their unique stability,superior biocompatibility,supramolecular and molecular recognition characteristics.Among gold nanomaterials with different morphologies,gold nanowires have important application potential in the field of biological detection due to their extremely high aspect ratio,good flexibility,and simple preparation methods.In this paper,the gold nanowire sensors are studied and simulated.Firstly,the principle of surface plasmon resonance generation is briefly described,and the detection mechanism of the combination of metal nanowire structure and surface plasmon resonance sensing technology is described in detail.Numerical calculation methods and analysis methods are briefly stated.Secondly,a nanowire-based surface plasmon resonance biosensor that can be used for DNA molecule detection is proposed.The occurrence and progression of diseases are closely related to the expression level of genomic DNA and the abundance of RNA in the cell.Directly used as the detection standard for some specific diseases.For the research of SPR sensor for DNA detection,enhancing the specific recognition ability of the DNA fragment probe on the surface of the SPR sensor is the focus of the research.Metal Nanowire Array SPR Biosensor for DNA Detection.The finite-difference time-domain algorithm is used to analyze the characteristics of the sensor.The effect of gold nanowires with different structural parameters on the sensitivity of the surface plasmon resonance(SPR)biosensor is optimized.DNA testing.Thirdly,this paper combines surface plasmon resonance technology with infrared spectroscopy technology,and proposes a multi-unit field-shaped metal nanowire array structure to achieve ?-Syn protein(causing neurodegeneration Detection of the secondary structure of Lewy body fibrosis protein).The sensor consists of several independent detection units with the same structure and layout.Due to the special field grid arrangement of the nanowire array,each detection unit has two sets of nanometers that can be excited by polarization waves with different polarization directions.The line detection array greatly increases the detection accuracy of the sensor in ultra-low concentration detection,and ensures that the actual area of the sensor is unchanged while increasing the detectable area without crosstalk.Finally,the detection of protein secondary structure in amide I band was simulated by finite-difference time-domain method.The dry thin-layer protein was simulated and analyzed,and the secondary structure of the protein was qualitatively detected by separating and collecting the overlapping components of the absorption signals in the reflection spectrum.The secondary structure information extracted from the protein was compared with the established protein model to achieve ?-Non-destructive and label-free detection of Syn protein secondary structure.Surface plasmon resonance(SPR)sensing,as a sensitive,real-time and label-free detection technology,provides mid-infrared(mid-IR)absorption spectra for protein secondary structure detection due to its powerful resonance near-field characteristics A new detection direction.
Keywords/Search Tags:Surface plasmon resonance, Photonic crystal fiber, Finite Element Method, Refractive index sensor, Local Surface Plasmon Resonance
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