Design And Implementation Of Microscopic Imaging System Based On Fourier Ptychographic Imaging Technology

Fourier ptychography is a novel technique for large field-of-view and high-resolution imaging.This technique mainly consists of three parts: structural lighting,laminar imaging and phase recovery.It means that under the imaging platform of the microscope,LED irradiated samples from different positions are imaged through the objective lens,and then CCD is used for low-resolution image acquisition.In the process of capturing pictures by the CCD,only the intensity image of the pictures are saved,and the phase information is lost during the acquisition process.By the means of Fourier ptychographic technique,a large number of low-resolution images are iterated and updated in the frequency domain,realizing the reconstruction of the large field-of-view,high-resolution intensity image and phase image of the sample.The purpose of this paper is to integrate the process of lowresolution image acquisition and complex amplitude information reconstruction in Fourier ptychographic microscopy.The fast reconstruction of sample intensity image and phase image is realized in a simple and convenient way.Firstly,based on the acquisition quality and efficiency of low resolution images,the LED light source array in the Fourier ptychographic microimaging device is designed and implemented,including the hardware circuit,embedded program and PC software of the LED light source array.The LED is controlled by the PC software through the WIFI module using the TCP/IP protocol.Secondly,the system of the Fourier ptychographic microscopic imaging is came true.The LED light source array,the ordinary optical microscope and the industrial camera are integrated,and the software program for controlling the LED is integrated with the phase recovery algorithm.When the program is running,the low-resolution image acquisition is performed by the microscopic imaging device,and the phase recovery algorithm is used to reconstruct the sample intensity image and phase image,and the integration of the Fourier ptychographic microscopic imaging system is successfully realized.Finally,through the two classical phase recovery algorithms of FP and TPWFP,three sets of experiments with aberration simulation,no aberration simulation and real data are performed respectively,The experimental results show that the three sets of experiments can reconstruct the high-resolution intensity image and phase image of the sample,and the subjective and objective comparisons of the FP algorithm in this experimental system are better than the TPWFP algorithm.The Fourier ptychographic microscopic imaging device built in this experiment is combined with the PC control software and phase recovery algorithm to realize the integration of the Fourier ptychographic microscopic imaging system.The system realizes the reconstruction of sample intensity and phase image simply and efficiently.