The Effect Of Aerobic Exercise On Alternative Splicing Of PKC?1 Pre-mRNA

Objective: To evaluate the effects of aerobic exercise on the alternative splicing of obesity-related gene PKC?1pre-m RNA in mice.Methods: C57BL/6 male mice were randomly divided control group(C),control + exercise group(C+E),obese group(O)and obese + exercise(O+E)group,in which group C and E were on normal diet,O and O+E group were on high-fat diet.After successful membrane formation,the E and O+E group were subjected to aerobic exercise for 8 weeks.Sacrifice immediately after exercise,the blood,liver,fat and other tissues were collected to detect biochemical indicators: mouse body weight,Lee's index,and blood lipids.Observation of lipid changes by Hematoxylin-eosin staining(HE staining).PCR and real-time PCR was used to determine the contents of PKC?1-FL,PKC?1-?Exon9 and SFRS10.Results:(1)After aerobic exercise,the body weight and Lee's index of obese mice decreased significantly,and total cholesterol(TC),triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)also showed a downward trend(P < 0.05),while high-density lipoprotein cholesterol(HDL-C)increased(P <0.05).(2)Hematoxylin-eosin staining showed that lipid droplets in fat cells became smaller after aerobic exercise.(3)The results of PCR and real-time PCR showed that the content of PKC?1-FL decreased after aerobic exercise,the content of PKC?1-?Exon9 increased,and the content of SFRS10 decreased.Conclusion:(1)Aerobic exercise can effectively reduce the body weight of obese mice.(2)Aerobic exercise can improve obesity in mice by altering the alternative splicing of the obesity-related gene PKC?1 pre-m RNA.(3)Aerobic exercise may alter the alternative splicing of the obesity-related gene PKC?1 pre-m RNA by affecting the splicing factor SFRS10,thereby improving obesity.