Effect Of Resistance Training On SIRT1/PGC-1?/NRF-1 Expression In Skeletal Muscle Of Type 2 Diabetic Mice

purpose:Effect of resistance training on insulin resistance,inflammatory response,muscle strength of skeletal muscle and wet weight of gastrocnemius muscle in type 2 diabetic mice;and the effect of resistance training on the expression of SIRT1 and PGC-1 in skeletal muscle of type 2 diabetic mice Obstructive training improves blood glucose metabolism and improves insulin resistance in type 2 diabetic mice.Methods:In the study,60 male C57BL/6J mice were used to produce type 2 diabetes model group by combining high fat diet and STZ:normal control group(NC),type 2 diabetes control group(DM),type 2 diabetes resistance training intervention group(DS),type 2 diabetes + SIRT1 agonist group(DR),type 2 diabetes + SIRT1 agonist +Resistance training intervention group(DRS).Exercise program:The use of ladders for resistance training,exercise intervention 3 times a week,each time 3*5 repetitions(climbing),the rest time between each group is 2 minutes.Eight weeks after intervention,blood samples were collected from all mice.Lipid metabolic parameters an?MDA and SOD levels were measured using the appropriate kits.Serum insulin,TNF-?,IL-10,and IL-6 levels were detected by ELISA.Serum levels were determined by CLIA.CRP levels;gastrocnemius muscles were isolated,wet weights were measured and protein expression levels of SIRT1,PGC-1?,and NRF-1 in gastrocnemius muscles were determined by Western Blot.Result:(1)fasting blood glucose and fasting insulin levels in mice fed with high-fat diet and STZ were significantly higher than those in NC group,and fasting blood glucose was more than 13.89mmol/L,which was regarded as successful;(2)8-week resistance training intervention significantly increased DM group The insulin sensitivity index effectively reduced the serum MDA,TNF-?,IL-6,CRP levels in the DM group,and increased the level of serum SOD,IL-10;(3)After 8 weeks of resistance training,the DS group loaded load and gastrocnemius muscle Wet weight increased significantly compared with DM group;(4)After 8 weeks of resistance training intervention,SIRT1,PGC-1?,and NRP-1 protein expression in skeletal muscle of DM group and DS group increased significantly;(5)SIRT1 agonist-treated DRS The level of inflammatory cytokines in the group was significantly lower than that in the DM group,and the insulin sensitivity was significantly higher than that in the DM group.At the same time,SIRT1 agonist can significantly increase the protein expression levels of PGC-1? and NRF-1 in skeletal muscle.Conclusion:(1)Resistance training improves muscle strength and increases the wet weight of gastrocnemius muscle in type 2 diabetic mice;resistance training improves insulin sensitivity and reduces inflammation in type 2 diabetic mice;(2)resistance training can upregulate type 2 SIRT1,PGC-1? and NRF-1 protein expression levels in skeletal muscle of diabetic mice.Resistance training may increase SIRT1,PGC-1?,and NRF-1 protein expression,enhance mitochondrial function,increase blood glucose metabolism,and improve insulin resistance in type 2 diabetic mice.