| Objective: It is to observe the expression effect for controlling proteins FOXO3 a,MAFbx32 and MURF1 on endurance exercise to the aging mice skeletal muscle atrophy and provides the experimental basis for endurance exercise to improve the aging sarcopenia.Method: The research is to construct the model of the aging skeletal muscle atrophy for the 11-month-old female C57BL/6 mice,which are randomly divided into a4-week quiet group(4C)and a 4-week exercise group(4T),a 8-week quiet group(8C)and 8-week exercise group(8T).4 C group and 8C group are quietly raised,4T group and 8T group are conducted the exercise of the moderate strength running platform at the same tine,with the speed of 15m/min,with the slope of the running platform O and the time of duration 60 min,five times a week.4C and 4T group put to death and got soleus after the 24 hours of the last training is ended;8C group and 8T group do the same as 4C and 4Tgroup.We have recorded mice body weight,soleus wet weight and HE dying observing cross sectional area.Western Blotting detects the expression of the controlling proteins FOXO3 a,MAFbx32 and MURF1 in skeletal muscle atrophy.Results:(1)Body weight: The mice body weight are showed downward trend during the exercising intervention in 4T group(28.97 ±1.68g),8C group(28.47±2.45g),8Tgroup(28.42±2.09g),which were decreased 7.5%,9.1% and 9.3% respectively,without significant difference;comparing with 4C group(31.33±3.51g).(2)The soleus muscle mass:(1)wet weight: The soleus muscle wet weight in4Tgroup(8.95 ± 1,05mg)and 8T group(8.27 ± 1.03mg)are showed rising trend without significant difference,comparing with 4C group(8.1 ± 0.72mg).(2)body weight: the body weight of 4T group(0.31 ± 0.05),8Cgroup(0.28 ± 0.03)and 8T group(0.29±0.04)have increased comparing with 4Cgroup(0.26±0.04);4T group comparing with 4C group has had in 4Tgroup(8.95±1,05mg)and 8T group(8.27±1.03mg)a significant difference(P<0.05).(3)Muscle Fiber Cross: comparing with 4C group(2095.7±225.6),the CSA of 4T group(2593.3 ± 49.1)and 8T group(2256.1 ± 86.7)have increased trend after exercising intervention,which are increased by 23.7%(P < 0.01)and 7.6%respectively.The 8C group(1921.4 ± 226.7)decreased by 8.3% comparing with4 C,meanwhile 8T group has increased by 17.4% comparing with 8C(P<0.01).(4)The expression of the skeletal muscle atrophy regulatory protein: FOXO3:comparing with 4C(0.44±0.26),4T(0.38±0.2)and 8T(0.43±0.15)have showed downward trend after exercising intervention without significant difference.But 8Cgroup(0.46 ± 0.06)has gradually risen comparing with 4C group.MAFbx32:comparing with 4C group(0.28±0.08),4T group(0.28±0.1)and 8T group(0.28±0.11)have showed no significant change after exercising intervention;the 8C group(0.44 ± 0.07)has significantly increased comparing with 4C group(P < 0.01);meanwhile 8T group has significantly showed decreased trend MURF1: comparing with 4C(0.32 ±0.16),4T(0.29 ±0.1)and 8T(0.3 ± 0.12)has showed decreasing trend after exercising intervention without significant difference and the expression of8 C group(0.39±0.2)has risen higher than that of 4C group.Conclusion:(1)The endurance exercising of4-week and 8-week have had a bit effect on the body weight of 11-month-old female C57BL/6 mice in the model of the natural aging mice skeletal muscle atrophy,but CAS is significantly higher than that of the same month-old mice and the soleus muscle mass is superior to the same age mice.(2)The possibility of the endurance exercising delaying the aging mice skeletal muscle atrophy developing mechanism may be: the endurance exercising can cause the expression decrease of FOXO3 a,further restraining the expression transcription of MAFbx32 and MuRf1,reducing the rate of skeletal muscle protein decomposition and playing an effect on delaying the skeletal muscle atrophy.The expression trend of MAfbx32 and MuRF1 are similar but the change of MURF1 iss not as obvious as that of MAFbx32.It indicates that it may be controlled by other protein signaling pathways and the effect is stronger than FOXO3a;furthermore,it is needed to do further experimental verification.(3)The intervention of the endurance exercising has had an active effect on delaying the development process of the aging skeletal muscle atrophy. |
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