Effects Of Eight Weeks Of Aerobic And Resistance Exercise On Fatty Liver Induced By In Mice

Research objectiveC57 mice were fed with high fat diet.After feeding,a group of C57 high sugar mice were randomly selected for eight weeks aerobic and anti exercise after the body was tested for pathological features.To observe the effect of exercise on liver metabolism and related gene expression in mice induced by high fat diet,and explain the effect of exercise on lipid metabolism disorder of liver induced by high fat diet.To provide the direction and evidence for the treatment and rehabilitation of fatty liver disease caused by high fat diet.Research methodThe experimental mice were 30 male C57 mice aged 8 weeks with an average weight of 20.02 ±0.06 grams.The experimental mice were from the Animal Experimental Center of Shanghai second military Medical University.The mice were randomly divided into C57 high fat diet group and C57 high fat diet group.The histopathological model of fatty liver was established by high fat diet feeding.During the course of diet intervention,IPGTT was tested weekly in mice.The fasting time interval was 8 hours.Blood samples were collected from caudal vein of mice at different time intervals,such as 60 mins,90 mins and 120 min of intraperitoneal injection of glucose.The blood glucose level of caudal vein was measured by blood glucose meter.The success of modeling was measured by calculating the area under the blood glucose curve.After the model was completed,the C57 mice were further divided into two groups:the quiet group with high fat and the exercise group with high fat.10 rats in each group had no change in diet composition and way.Research results1.Compared with the low fat quiet group,the mice in the high fat quiet group showed a very significant difference(P<0.01),compared with the high fat quiet group(P<0.01),and the area under the blood glucose curve was significantly different between the high fat quiet group and the high fat quiet group.In the hyperlipidemic exercise group,there was a very significant difference in the area under the blood glucose curve between the two groups(P<0.05).2.After two weeks of high fat diet feeding,the body weight of the quiet group was significantly higher than that of the rest group with low fat diet,and the p value of the rest group was less than 0.05.After two weeks of exercise training,the mice were fed with high fat diet.The body weight of high fat exercise group was significantly lower than that of high fat quiet group(P<0.05).Compared with low fat quiet group,the liver weight and liver/body weight of high fat quiet group increased significantly than that of low fat quiet group,but lower than that of high fat quiet group,while that of high fat quiet group was lower than that of high fat quiet group.The liver weight and liver/body weight of high fat exercise group were lower than 0.05%(P<0.05).3.The elevation of triglyceride,total cholesterol,high density lipoprotein,low density fat egg and free fatty acid in high fat quiet group was lower than that in low fat quiet group,while that in high fat exercise group was lower than that in high fat quiet group.There was no significant difference in high density lipoprotein and low density fat egg,but the free fatty acid decreased,and the difference was significant(P<0.05).4.Compared with the low fat quiet group,the liver AST(aspartate aminotransferase)and alt(alanine aminotransferase)in the high fat quiet group were higher than those in the low fat quiet group.There was significant difference(P<0.01)^ and the alt(alanine aminotransferase)in high fat exercise group was lower than that in quiet high fat group(P<0.05).5.Oil red staining showed that the nucleus was old color and fat was red.The high fat quiet group had a lot of fat tissue compared with the low fat quiet group,but the high fat exercise group was compared with the high fat exercise group.The results of glycogen staining showed that glycogen deposition appeared in the liver of high fat quiet group,while that in high fat exercise group was alleviated.6.Western blot test showed that compared with low fat quiet group,The expression of PGC1 a in the liver tissue of the hyperlipidemic quiet group was higher than that of the control group(P<0.05)and the expression of PPAR a was lower than that of the control group(P<0.05).The P value of the hyperlipidemic exercise group was lower than that of the control group(P<0.05),and that of the hyperlipidemic exercise group was lower than that of the high-fat quiet group(P<0.05).In exercise group,the level of PGC1α expression decreased and the P value was less than 0.05.The increase of PPAR a expression level was less than 0.05 in the exercise group.The decrease of the phosphorylation expression of PGC1 a in the exercise group was lower than that in the PPAR a phosphorylated water,and the P value was less than 0.05 in the exercise group.Research conclusion1.24 weeks of high fat diet can establish a pathological model of fatty liver.High fat diet mice will have high insulin,hyperlipidemia,liver glucose and lipid metabolism disorder and mitochondrial dysfunction.2.8 weeks of oxygen combined with anti resistance exercise can improve the hyperinsulinemia,hyperlipidemia,liver glucose and lipid metabolism disorder and mitochondrial dysfunction,and improve the liver function.3.The expression of GCN2,P-AKT,P-EIF2 alpha,PGC1-alpha and PPAR alpha changed,liver fatty acid uptake increased and oxidative utilization ability decreased,liver mitochondria dysfunction.4.Exercise can regulate the expression of P-AKT,P-EIF2,PGC1-and PPAR,and improve lipid accumulation and mitochondrial dysfunction in liver.