Expression Of GS28 And Its Relationship With Prognosis In Endometrial Carcinoma

ObjectiveThis study was designed to evaluate the expression and subcellular localization of GS28 in endometrial carcinoma,and compare the expression differences of GS28between normal endometrium tissue,endometrial hyperplasia without atypia tissue,endometrial atypical hyperplasia tissue and endometrial carcinoma tissue,and to investigate the correlation between the nuclear expression of GS28 and clinicopathological indexes and prognosis of endometrial carcinoma,and we also analyzed the correlation between GS28 expression and ER,PR,p53 and Ki67.Then we will explore the role of GS28 nuclear expression in the occurrence and development of endometrial carcinoma and its relationship with prognosis.MethodA total of 87 cases of pathological tissue specimens were selected from the First Affiliated Hospital of Jinan University from October 2012 to May 2018,including 46cases of endometrial carcinoma,14 cases of endometrial atypical hyperplasia,12cases of endometrial hyperplasia without atypia and 15 cases of normal endometrium in the control groups.Immunohistochemical staining was used to detect the expression level of GS28 in endometrial tissues of each group,and Western blotting method was used to detect the subcellular localization of GS28 in endometrial carcinoma tissues and normal endometrial tissues.Statistical methods were used to analyze the relationship between the expression intensity of GS28 and the degree of endometrial lesion,the correlation between the expression of GS28 nucleus and the clinicopathological features of patients with endometrial carcinoma,and the correlation between the expression of GS28 nucleus and the expression of ER,PR,p53 and Ki67 in endometrial carcinoma.46 patients with endometrial carcinoma were followed up.The relationship between GS28 nuclear expression and progression free survival time and overall survival time was analyzed by Kaplan-Meier log-rank test,and the survival curve was drawn.Finally,we used COX proportional hazard regression model to analyze the correlation between GS28 nuclear expression and prognosis of patients with endometrial carcinoma.Result1.With the increase of disease grade of normal endometrium,endometrial hyperplasia without atypia,endometrial atypical hyperplasia and endometrial carcinoma tissue,the expression intensity of GS28 gradually increased.The difference was statistically significant(P=0.00,r_s=0.069).2.GS28 was localized at nucleus and cytoplasm in endometrial carcinoma tissue,but only at cytoplasm in normal endometrium,endometrial hyperplasia without atypia and endometrial atypical hyperplasia tissue.There was significant difference in the expression of GS28 in the nuclei of four kinds of tissues(P=0.00).3.In endometrial carcinoma,the nuclear dominant expression of GS28 was correlated with FIGO stage and depth of myometrial invasion(P<0.05),but not with age,histological grade,vascular tumor thrombus,lymph node metastasis and histological type(P>0.05).4.There was no significant correlation between nuclear dominant expression of GS28 and ER,PR,p53 and Ki67(P<0.05).5.Kaplan-Meier showed that the nuclear expression of GS28 was related to the progression free survival rate and overall survival rate of patients with endometrial carcinoma(P=0.00).The progression free survival time and overall survival time of patients with nuclear dominant expression of GS28 was significantly lower than that of patients with non-dominant expression of GS28,suggesting that GS28 nuclear dominant expression is related to poor prognosis of patients with endometrial carcinoma.6.The univariate analysis showed that GS28 nuclear expression,histological grade,depth of myometrial invasion,vascular tumor thrombus,lymph node metastasis histological type and postoperative radiotherapy were all related to the postoperative prognosis of patients with endometrial carcinoma.COX multivariate analysis showed that GS28 nuclear expression was independent predictors of postoperative prognosis in patients with endometrial carcinoma.Conclusion1.With the gradual evolution of normal endometrium,endometrial hyperplasia without atypia,endometrial atypical hyperplasia and endometrial carcinoma,the expression intensity of GS28 increased gradually,suggesting that GS28 is involved in the whole process of the occurrence and development of endometrial carcinoma.2.Nuclear localization of GS28 was found in endometrial carcinoma.With the increase of FIGO stage and the depth of myometrial invasion,the nuclear expression of GS28 was gradually up-regulated,suggesting that the nuclear dominant expression of GS28 may play a positive regulatory role in the progression and metastasis of endometrial carcinoma.3.The lower the progression free survival rate and overall survival rate of patients with GS28 nuclear dominant expression is,the worse the clinical prognosis is.It is suggested that the nuclear expression of GS28 may be an independent predictor of prognosis in endometrial carcinoma.