| Objectives:1.To study the role of IL-22 in the pathogenesis of acute gout arthritis.2.To study the relationship between single nucleotide polymorphism of LRRC16A gene and the risk of acute gouty arthritis preliminarily.Methods:1.ELISA was used to detect the peripheral blood IL-22 concentrations of 77 patients with acute gouty arthritis(AGA)(68 males,9 females)and 30 healthy controls(HC)(20males,10 females),and the data were recorded.Peripheral blood uric acid,blood glucose,serum creatinine,24 hours urinary uric acid,24 hours urinary creatinine,cereal third transaminase(ALT),aspertate aminotransferase(AST),cholesterol,triglyceride,low density lipoprotein cholesterol(LDL-c),high-density lipoprotein cholesterol(HDL-c),the estimated glomerular filtration rate(eGFR),high-sensitivity c-reactive protein(hsCRP)and erythrocyte sedimentation rate(ESR)were detected in AGA patients.The difference of peripheral blood IL-22 levels between the patients with acute gouty arthritis and the healthy control group was analyzed,and the correlation between peripheral blood IL-22 concentrations and other clinical indicators in the case group was evaluated.2.LRRC16A gene loci rs2149228 and rs742132 of 30 cases of patients with acute gouty arthritis(AGA)(30 males)and 30 cases of healthy controls(HC)(30 males)were detected by using the technique of polymerase chain reaction and the generation of gene sequencing technology.The relationship between rs2149228 and rs742132 in LRRC16A gene single nucleotide polymorphisms and the risk of acute gouty arthritis was analyzed preliminarily.3.Statistical methods:K-S was used to check whether the measured data met the normal distribution,and(mean±SD)means mean plus or minus standard deviation),t test was used for comparison of measurement data between two groups,and variance analysis was used for comparison of measurement data among multiple groups.Pearson linear correlation analysis was used for correlation analysis.Chi-square test of goodness of fit was used for the frequency distribution of genotypes.Results:1.There was no statistically significant difference in age and sex between the 77 patients with acute gout arthritis and the 30 healthy controls,and the two groups were comparable.The peripheral blood IL-22 concentration of gout patients was higher than that of the healthy control group,which was(7.95±5.52)ng/L?(5.20±2.57)ng/L,respectively.The difference between the two groups was statistically significant(t=-2.62,P=0.01,see table 2).2.There was no correlation between peripheral blood IL-22 concentration and blood glucose,serum uric acid level,serum creatinine level,eGFR,ALT,AST,total cholesterol,triglyceride,low density lipoprotein,high density lipoprotein,24-hour urinary uric acid,24-hour urinary creatinine and BMI of 77 patients in the AGA group(P>0.05,see table 3).The peripheral blood IL-22 concentration of patients in the AGA group was positively correlated with the level of hsCRP,and the peripheral blood IL-22 concentration level of patients in the AGA group was also positively correlated with the level of ESR(P<0.05,see table 3).3.The distribution of genotype frequency in the healthy control group was analyzed by Chi-square test of goodness of fit.The results showed that the frequency distribution of the rs2149228 genotype of LRRC16A gene was consistent with Hardy.Weinberg equilibrium law and was representative of the population(P>0.05).By comparing the genotypes and alleles of rs2149228 in LRRC16A of two groups,we found that the AT genotype frequency of rs2149228 was the highest,followed by the AA genotype and the TT genotype.rs2149228 genotype frequency of control group(AA,30%;AT,70%;TT,0%)and gout group(AA,43.3%;AT,50%;TT,6.7%)showed no statistically significant difference(P>0.05),and there was no statistically significant difference between two groups in allele frequencies of A and T(P>0.05,see table 6).4.The distribution of genotype frequency in the healthy control group was analyzed by Chi-square test of goodness of fit.The results showed that the frequency distribution of the rs742132 genotype of LRRC16A gene was consistent with Hardy.Weinberg equilibrium law and was representative of the population(P>0.05).rs742132 of LRRC16A gene.It was found that the AA genotype of rs742132 had the highest frequency,followed by the AG genotype,and no GG genotype was found in healthy controls.The frequency of AA genotype was equal to that of AG genotype in the gout group.There was no statistically significant difference of rs742132 genotype frequency in LRRC16A gene between the healthy control group(AA,70%;AG,30%;GG,0%)and acute gouty arthritis group(AA,50%;AG,50%;GG,0%)(P>0.05),and the difference in allele frequencies of A and G between the two groups was not statistically significant(P>0.05,see table 7).Conclusions:1.Increased peripheral blood IL-22 concentration in patients with acute gout arthritis was positively correlated with ESR and hsCRP,suggesting that IL-22 may be associated with the incidence of acute gout arthritis.2.AT genotype frequency of rs2149228 was the highest,followed by AA genotype and the lowest TT genotype in the gout group and the control group.Genotype frequency of rs2149228 showed no statistically significant difference between the two groups(P>0.05),and there was no statistically significant difference in allele frequencies of A and T(P>0.05).3.AA genotype of rs742132 had the highest frequency,followed by the AG genotype,and no GG genotype was found in healthy controls.The frequency of AA genotype was equal to that of AG genotype in the gout group and no GG genotype was found.There was no statistically significant difference of rs742132 genotype frequency in LRRC16A between two groups and the difference in allele frequencies of A and G was not statistically significant(P>0.05). |
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