Analysis Of Biomakers Related To Gastric Cancer Progression And Prognosis By Bioinformatics Screening

Objective: Abnormal gene expression is an established cause of gastric cancer(GC)initiation and progression.In this study,we aimed to identify several key genes that could be used to effectively predict progression and prognosis in patients with GC.Methods: First,the Linked Omics database was used to analyze the data from the TCGA database,and select genes that are significantly positively correlated with the pathological stage of gastric cancer,the number of metastatic lymph nodes and overall survival.Then GEO2 R online was used to analysis the expression of the selected genes in gastric cancer and corresponding adjacent tissues in the GEO data set.Genes were selected whose expression is significantly up-regulated in gastric cancer tissues.Real-time fluorescent quantitative PCR and Western Blotting were used to verify the expression levels of the screened genes from the m RNA and protein levels in tissue samples.And Kaplan-Meier survival analysis was used to predict the relationship between candidate genes and the overall survival and progression-free survival of gastric cancer patients.Finally,the CCK8 experiment was used to verify the selected genes at the cellular level.Results: By analyzing the data of gastric cancer in the TCGA database,14 genes that are significantly positively correlated with the pathological staging,number of metastatic lymph nodes and overall survival of patients with gastric cancer were selected.Analyzed the expression of 14 candidate genes in the gastric cancer tissue and adjacent normal tissues in the microarray which downloaded from the GEO database,respectively,GSE118916 and GSE54129.The results showed that the expression of MYO5 A,TPP1,TGFBR2,PALM2-AKAP2 and PLTP in gastric cancer tissue both are higher than the adjacent tissues.Real-time fluorescent quantitative PCR and Western Blotting confirmed that the expression of MYO5 A,PLTP and TPP1 in gastric cancer tissues was significantly higher than that in adjacent normal tissues.Kaplan-Meier survival analysis showed that MYO5 A,PLTP and TPP1 were positively correlated with the overall survival and progression-free survival of patients with gastric cancer.The results of CCK8 experiment found that MYO5 A and PLTP could promote the proliferation of gastric cancer cells in vitro.Conclusion: In this study,it was found that the expression of MYO5 A,PLTP and TPP1 in gastric cancer tissues was higher than that of adjacent normal tissues.The high expression of MYO5 A,PLTP and TPP1 was positively correlated with the progression and prognosis of gastric cancer.In vitro,we also confirmed that MYO5 A and PLTP can promote the proliferation of gastric cancer cells.This study provides a theoretical basis for MYO5 A,PLTP and TPP1 as biomarkers related to early diagnosis and progression of gastric cancer.