Study On Quality Standards Of Celosiae Semen

It is a dry mature seed of Celosia argentea L.,which is oblate and round,black and can be used as medicine.It is harvested in autumn.The medicinal record of the Celosiae Semen begins with the "Compendium of Materia Medica" and has the effect of clearing the liver and clearing the eye.It is used as a medicine for treating liver damage and eye diseases.It has been included in many classic compounds.Because the appearance of Celosiae Semen is ordinary,there is no special point.Therefore,there are many fake products on the market.The current quality standards only have microscopic,trait identification,impurity inspection items,etc.,lack of thin layer identification and content determination,etc.Based on thin-layer chromatography and high-performance liquid chromatography,this paper studies the medicinal materials of Celosiae Semen,in order to improve the quality standards of Celosiae Semen and improve the content of pharmacopoeia.1 Identification of Celosiae Semen by thin layer chromatographyBy thin-layer chromatography,this experiment established a thin layer identification method for the Celosiae Semen.The conditions for thin-layer chromatography were: n-butanol-acetic acid-water(4:1:1);the developer was 10% sulfuric acid.Ethanol;room temperature.The method can clearly distinguish the three index components of celosin I,celosin J and celosin H on the thin layer plate,and can effectively identify the Celosiae Semen and its common fakes.2 Fingerprint study of Celosiae SemenIn this experiment,an electrospray detector(CAD)was used to establish a fingerprint method for the Celosiae Semen medicinal materials,which was calibrated.The 16 common peaks were compared with the pseudo-liquid phase spectra.This method can effectively identify the Celosiae Semen and its common counterfeit,and the similarity range is between 0.935 and 0.999.The liquid phase gradient elution method is: 0-15 min,25%-28% acetonitrile;15-19 min,28%-26% acetonitrile;19-25 min,26%-30% acetonitrile;25-35 min,30%-40% Acetonitrile;35-42 min,40%-55% acetonitrile;42-50 min,55% acetonitrile;50-52 min,55%-95% acetonitrile;52-62 min,95% acetonitrile.Instrument precision,method repeatability,durability,and sample stability are good.3 Determination of celosins in Celosiae Semen by HPLC-CADIn this experiment,the HPLC-CAD content determination method of celosin I,celosin J and celosin H was established by electrospray detector(CAD).The chromatographic conditions were as follows: Waters CORTECS C18(4.6*150 mm,2.7 ?m)as the column;mobile phase: acetonitrile-0.3% aqueous glacial acetic acid;flow rate: 1 ml/min;column temperature: 30 ° C;CAD detector atomization temperature: 50°C.Gradient elution: 0-15 min,25%-28% acetonitrile;15-19 min,28%-26% acetonitrile;19-25 min,26%-30% acetonitrile.The celosin I was linear in the range of 0.01009mg/ml-0.2018mg/ml,and the celosin J was linear in the range of 0.000857mg/ml-0.1714mg/ml,and the celosin H was 0.005012mg/ml-0.2506mg/ml There is a linear relationship in the range of 0.2506 mg/ml.The detection limits of celosin I,J,and H were: 1 ng,0.6 ng,and 0.5 ng.The limit of quantification of celosin I,J,and H were 4 ng,2.6 ng,and 3 ng,respectively.Instrument precision,method repeatability,Durability and sample stability are good.4 Determination of celosins in Celosiae Semen by HPLC-ELSDIn this experiment,an HPLC-ELSD content determination method for celosin I,celosin J,and celosin H was established by using an evaporative light scattering detector(ELSD).The chromatographic conditions were as follows: Waters CORTECS C18(4.6*150 mm,2.7 ?m)as the column;mobile phase: acetonitrile-0.3% aqueous glacial acetic acid;flow rate: 1 ml/min;column temperature: 30°C;ELSD detector parameter evaporation temperature: 110°C;atomization temperature: 90°C.Gradient elution: 0-15 min,25%-28% acetonitrile;15-19 min,28%-26% acetonitrile;19-25 min,26%-30% acetonitrile.The celosin I was linear in the range of 0.01009mg/ml-0.2018mg/ml,and the celosin J was linear in the range of 0.000857mg/ml-0.1714mg/ml,and the celosin H was 0.005012mg/ml-0.2506mg/ml There is a linear relationship in the range of 0.2506 mg/ml.The detection limits of guanosine I,J,and H were: 0.0404 ?g,0.0857 ?g,and 0.0451 ?g,respectively.The quantitative limits of celosin I,J,and H were 0.0959 ?g,0.1714 ?g,and 0.1128 ?g,respectively.Instrument precision,method repeatability,durability,and sample stability are good.