Separation And Analysis Of Bisphosphonates By Reverse Phase High-performance Liquid Chromatography

Bisphosphonates are bone resorption inhibitors drugs, which had been widely used in the management of skeletal disorder including osteoporosis, malignant hypercalcemia, bone metasis and Paget's disease.It is difficult to find a chromatographic system for bisphosphonates and related substances, which are strongly polary and hard to retain on non-polar stationary phase such as C₈ or C₁₈ column. Morever, development of a chromatography assay for this class of compounds is challenging owing to lack of a chromphore for conventional UV or fluorescence detection. Bisphosphonates cannot be analyzed easily with GC, because of their low volatility and polar nature. Few bisphosphonates such as alendronate, pamidronat should be determined by UV or fluorescence detection based on derivatization, but the procedure is complex, time-consuming, or their derivatives are unstable. IEC methods for bisphosphonates usually used anion-exchange column that can stand strong acid as the stationary phase, the detection systems are not common in most analytical laboratories.The objective of our research is to establish a...