Effects Of Human Cytomegalovirus Infection On Anemia In Children And Analysis Of Related Immune Indexes

Purposes:To investigate the effect of human cytomegalovirus(HCMV)infection on anemia in children and analyze the related immune indexes.Methods:From February 2018 to October 2019,80 cases of HCMV active infection diagnosed in Jiangxi Children's Hospital,aged 6 months to 6 years old,were selected to collect their clinical data and related auxiliary test reports.Another 80 cases of normal control group were taken from the children examined by the department of child health care of Jiangxi children's hospital,excluding HCMV infection,aged between 6 months and 6 years old.Urine or serum HCMV-DNA was detected by Polymerase Chain Reaction(PCR),anticoagulant hemoglobin(Hb)content was detected by hematology analyzer,CD4~+T percentage,CD8+T percentage,CD4~+T/CD8~+T ratio and NK cell CD16~+CD56~+percentage were detected by flow cytometry.The contents of serum IL-17 and IFN-?were detected by ELISA.Results:1.Comparison of Hb value and anemia incidence between 1.HCMV infection group and control group1.1 Hb(113.06±16.17g/L)in HCMV infection group was lower than that in control group(128.26±10.04 g/L),the difference was statistically significant(P<0.05).1.2 the incidence of anemia in HCMV infection group(33.75%)was significantly higher than that in control group(3.75%),the difference was statistically significant(P<0.01).2.Comparison of CD4~+T lymphocyte percentage,CD8~+T lymphocyte percentage,CD4~+T/CD8~+T ratio and NK cell percentage between HCMV infection group and control group2.1 the percentage of CD4~+T lymphocytes in HCMV infected group(21.66±4.55%)was lower than that in control group(33.81±4.17%),with statistically significant difference(P<0.05).2.2 the percentage of CD8~+T lymphocytes in HCMV infected group was(19.71±3.34%)lower than that in control group(23.23±4.86%),with statistically significant difference(P<0.05).2.3 the ratio of CD4~+T/CD8~+T in HCMV infection group(1.09±0.099)was lower than that in control group(1.49±0.201),with statistically significant difference(P<0.05).3.Comparison of NK cell percentage between HCMV infection group and control groupthe percentage of NK cells in HCMV infected group was(35.43±5.02%)higher than that in control group(18.32±3.76%),the difference was statistically significant(P<0.01).4.Comparison of IL-17 and IFN-?contents between HCMV infection group and control group4.1 the content of IL-17(38.86±3.56pg/mL)in HCMV infection group was significantly higher than that in control group(17.63±2.43pg/mL),with significant difference(P<0.01).4.2 the IFN-?content(28.74±2.34pg/mL)in HCMV infection group was significantly higher than that in control group(14.58±1.67pg/mL),the difference was statistically significant(P<0.01).5.Correlation between HCMV-DNA Load and Hb in HCMV Infected GroupThere was no correlation between HCMV-DNA load and Hb value in HCMV infection group(r=-0.313,P>0.05)Conclusios:1.HCMV infection can cause anemia in children.Anemia is relatively high in children with active HCMV infection.2.HCMV infection in children can cause changes in T lymphocyte subsets.The percentages of CD4~+T lymphocyte,CD8~+T lymphocyte and CD4~+T/CD8~+T ratio are lower than those in the control group,indicating that HCMV infection in children affects the balance of T lymphocyte subsets.3.The percentage of NK cells infected with HCMV in children increased,indicating that NK cells play an important role in children's anti-HCMV infection.4.IL-17 and IFN-?are highly expressed in HCMV infected children,suggesting that IL-17 and IFN-?can mediate inflammatory reaction in bone marrow hematopoietic microenvironment and damage hematopoietic cells.5.The infection of HCMV in children causes the immune activation of the body.Under the joint action of immune cells and cytokines,it causes the disorder of erythroid hematopoietic function and anemia.