Clinical And Experimental Study On The Diagnosis And Treatment Of Renal Cancer Based On The Theory Of Treating Both Symptoms And Root Causes

Objective This study analyzes the distribution of clinical TCM syndromes in renal cancer patients and explores its correlation with common risk factors such as drinking history,body mass index of kidney cancer patients(BMI),smoking history,drinking history,hypertension history,family history,etc.And the purpose of combining prevention and treatment;further constructing a tumor-bearing mouse model of kidney cancer disease,exploring the effect and possible mechanism of Ruanjian Sanjie Recipe on inhibiting the tumor growth of 786-0tumor-bearing mice,and providing rigorous experiments for Ruanjian Sanjie prescription against RCC Basis to provide new ideas for clinical treatment of RCC.Methods1.Research on the distribution rules of TCM syndromes of kidney cancer patients and their correlation with common risk factors.A retrospective survey should be used to develop a clinical symptom evaluation scale for patients with kidney cancer,establish a database of information about the four diagnosis of patients,and statistically analyze the TCM syndromes of 145 patients with kidney cancer.Type distribution law.Multivariate logistic regression was used to analyze the main TCM syndrome types and gender,age,pathological type,long-term quality of life(KPS)score,family history,hypertension history,smoking history,drinking history,BMI and other common risk factors.2.Ruanjian Sanjie Recipe inhibits tumor growth of 786-0 tumor-bearing nude mice and its possible mechanism.By constructing 786-0 tumor-bearing mouse model,it is divided into model group,high-dose group of Ruanjian Sanjie prescription,There were 6 mice in each group,low-dose group,?-secretase inhibitor(DAPT)group and LY294002 group.Before each administration,observe the growth status of nude mice and weigh the body mass and tumor volume of nude mice,draw the growth curve of subcutaneous tumors,calculate the tumor growth inhibition rate,and detect the expression levels of key apoptosis proteins BCL-2 and Bax by immunohistochemistry.Results:1.The distribution pattern of TCM syndromes in kidney cancer patients and its correlation with common risk factors The TCM syndromes in kidney cancer patients mainly include spleen and kidney qi deficiency syndrome 38.6%,liver and kidney yin deficiency syndrome 15.9%,spleen and kidney yang deficiency syndrome 13.1%,damp heat The betting card is mainly 9.7%.Drinking history and BMI?24kg / m2 are the risk factors of spleen and kidney qi deficiency syndrome;smoking history and drinking history are the risk factors of damp heat syndrome;hypertension history is the protective factor of liver and kidney Yin deficiency syndrome;Cardioprotective factors,age is a protective factor for spleen and kidney yang deficiency syndrome.2.Ruanjiansanjiefang inhibits tumor growth of 786-0 tumor-bearing nude mice and its possible mechanism2.1 General condition of nude mice The body weight of each group of nude mice decreased first and then increased.Some nude mice suffered from mental insufficiency,slow movement,sudden weight loss,etc,and later died.2.2 Suppression rate of the tumor High,medium and low dose group,DAPT group,LY294002 group,the tumor suppression rate is 46.26%,31.89%,24.06%,24.83%,26.91%(P <0.05)The dosage group can inhibit the growth of tumor-bearing mice,and the high-dose group of Ruanjiansanjiefang has the best tumor suppression effect.2.3 Comparison of key protein levels Bax IHS scores of the Ruanjiansan high-dose group,DAPT group,and LY294002 group were significantly higher than the model group,and Bcl-2 IHS scores were lower than the model group,suggesting the administration group Both can up-regulate Bax and down-regulate the expression level of Bcl-2(P <0.05).Conclusion1.Common clinical syndromes of kidney cancer patients mainly include spleen and kidney qi deficiency syndrome,liver and kidney yin deficiency syndrome,spleen and kidney yang deficiency syndrome,and damp heat bet syndrome,which are related to drinking history,BMI?24kg / m2,smoking history Sex.2.Ruanjian Sanjie Recipe can inhibit the growth of tumor-bearing mice,which may be achieved by up-regulating the expression of Bax and down-regulating the expression of Notch1,Akt,and Bcl-2.Notch1 and Akt may be the anti-tumor effect of Ruanjian Sanjie Recipe New target.