Study On The Relationship Between MiRNA-671-3p And CKAP4 On The Proliferation Of Glioma Cells

BACKGROUND AND OBIECTIVE:Human glioma is widely recognized as a malignant tumor of the nervous system originated from human glial cells.it is more common in China and has a high degree of malignant recurrence.The higher the clinicopathological grade of human glioblastoma,the stronger its ability to damage and invade the surrounding normal brain tissue,so it can not be completely removed before or during the operation,which may lead to malignant recurrence after operation.it is easier to develop to a higher malignant grade tumor.Human glioblastoma,is considered to be the most malignant tumor in human glioblastoma at present.At present,it is considered that the best way to treat glioblastoma is to treat glioma first,and then give patients combined radiotherapy and chemotherapy.However,most of the patients with glioblastoma malignant tumor after the above treatment still can not achieve a better treatment and prognosis.With the development of anticancer drugs and the proposal and introduction of precision medicine,the protein structure and molecular composition related to exocrine,mirco-RNA and core receptors are deeply analyzed and studied,as well as the attention and pursuit of neurosurgical experts and doctors for non-invasive or minimally invasive diagnosis and treatment of glioma,the clinical research of glioma has also entered a new era.As a hot spot of tumor research in recent years,micro-RNAs is widely considered to be closely related to the progress of tumor cells associated with core receptors and the possibility of cancer treatment.Because microRNAs is involved in signal transduction,gene expression plays a role in transcription and regulation of tumor cells,so microRNAs and its core receptors have become a promising target for anticancer drugs targeting glioma therapy.The current research results of mirco-671 have found that it is closely related to the early occurrence and development of many tumor diseases.Many studies have shown that mirco-671 can regulate tumor cells through the signal transduction of wnt core receptors activated by tumor cells,so as to promote(or inhibit)tumor cell migration and tumor cell invasion.At present,it has been further confirmed thattumor dickkopf1 protein(dkk1)antagonizes carcinogenic Wnt signal by binding to tumor wnt core receptor and low density lipoprotein core receptor related protein6.Cytoskeleton related protein 4 can regulate tumorigenesis by participating in a variety of signal pathways.Some studies have found that DKK1 binding CKAP4 and DKK1 tuberculosis LRP6 have similar affinity,based on the above research basis.The purpose of this study is to explore whether miRNA-671-3p and CKAP4 play an important role in the occurrence and development of human gliomas,and to further explore whether there is a direct targeting relationship between mir-671-3p and CKAP4,so as to provide a new targeted therapy for clinical treatment of human gliomas.METHODS:The expression of cytoskeleton associated protein 4 was detected by Western blotting Western blot,the expression of miR-671-3p was detected by immunofluorescence quantitative PCR,the proliferation activity of glioma cells was detected by cell counting kit-8,and the target gene of miR-671-3p was determined by luciferase method.RESULTS:The results showed that the expression of CKAP4 was significantly decreased and the level of mir-671-3p was significantly increased in human glioma tissues.In cell experiment,the overexpression of miR-671-3p could promote the proliferation of tumor cells in vitro,and the proliferative activity of tumor cells decreased by inhibiting the expression of miR-671-3p.CKAP4 was identified as the direct target of miR-671-3p by luciferase method.CONCLUSION:Through experiments,it is found that miR-671-3p can positively regulate the proliferation of human glioma in vitro,and there is a targeted relationship between miR-671-3p and CKAP4.CAKP4 can be used as a direct target of miR-671-3p to regulate the development of glioma cells,so we can consider miR-671-3p and CKAP4 as potential targets for glioma therapy.