Study On Extraction And Anti-tumor Activity Of Juglanone From Walnut

Juglans mandshurica is rich in resources in China.Juglans quinone in Juglans mandshurica has been proved by some studies not only to have significant hemostatic and antibacterial functions,but also some in vivo and external experiments have shown that it can also play a role in the field of antitumor.However,the molecular mechanism of this anti-tumor effect is still unclear.It is of great significance to study the genetic changes of gastric cancer cells under the intervention of walnut quinone for its clinical practice in the treatment of gastric cancer and to reveal the molecular biological mechanism of walnut quinone's anti-tumor effect.In this paper,walnut quinone was obtained by ethanol ultrasonic extraction,and its structure was identified by nuclear magnetic resonance.The CCK-8 method was used to screen the most sensitive strains to study the anti-tumor mechanism of walnut quinone.In human cervical cancer cells(HELA),human gastric cancer cells(SGC-7901),human liver cancer cell lines(HeP-2),human Lung adenocarcinoma cell line(A549),breast cancer cell line(MCF7),human neuroblastoma cells(SH-SY5Y-P9)were screened,the experimental results showed that jugoquinone has a certain inhibitory effect on 6 types of tumor cells Among them,human gastric cancer cells(SGC-7901)are the most sensitive cells among the six types of tumor cells.Therefore,this experiment used human gastric cancer cells(SGC-7901)as the research object to screen for differential genes.Through bioinformatics analysis of the high-throughput sequencing data,we finally screened 4248 differentially expressed genes of human gastric cancer cells(SGC-7901)treated with walnut quinone and control cells,of which 2364 differential genes were significantly up-regulated,1884 The differential genes were significantly down-regulated.The ten up-regulated differential genes with the largest Fold Change changes in differential gene analysis are: AL133352.1,TRIM39-RPP21,MMP23 A,MTND4P12,AC016717.1,SPATC1,AC008735.1,AC010442.2,PCDH17,CR559946.2;Fold Change The 10down-regulated differential genes with the largest.changes are: SPI1,AC148477.5,RPS10-NUDT3,GAGE12 J,PINCR,TNFSF12-TNFSF13,INO80B-WBP1,ISY1-RAB43,CSF3 R,AC005154.6.Further enrichment analysis of KEGG signaling pathways showed that these differential genes were mainly enriched in alcoholism signaling pathway,Hippo signaling pathway,Apelin signaling pathway and p53 signaling pathway.Finally,throughWestem-blot and RT-PCR detection,we found that the expression level of p53 protein in human gastric cancer cell(SGC-7901)apoptosis-related gene was significantly up-regulated after the action of walnut quinone;different concentrations of walnut quinone induced human gastric cancer cells(SGC-7901)The expression level of p53 gene mRNA was significantly enhanced after apoptosis,and it was dose-dependently related to the concentration of walnut quinone.The results of this study confirmed that the mechanism by which walnut quinone causes apoptosis of human gastric cancer cells(SGC-7901)may be induced by the induction of apoptosis,and is related to the regulation of the expression of apoptosis-related gene p53.This study is a clinical use of walnut quinone target It provides a certain scientific basis for the method of inhibiting the growth of tumor cells.