Studies On The Antitumor Effect Of Modified Xuezheng Decoction On Human Gastric Carcinoma Cells SGC-7901 And Its Related Mechanism

Purpose:We sought to observe the influence of modified Xuezheng decoction on the proliferation and growth of human gastric cancer cells SGC-7901. Meanwhile, we also aimed to explore the related mechanism.Methods:1. The proliferation inhibitory effect of the modified Xuezheng decoction on different concentrations of human gastric cancer cell line SGC-7901 cultured in vitro and its correlation with time were assayed by MTT.2. The anti angiogenic effect of proliferation of chick embryo chorioallantoic membrane technology and in vitro validation in the modified Xuezheng decoction.3. The influence of the modified Xuezheng decoction on gastric cancer cell line SGC-7901 apoptosis was detected by Annexin V/PI double staining fluorescence.4. The effect of the modified Xuezheng decoction on the cell cycle of different concentrations of gastric cancer cell line SGC-7901 was tested flow cytometry.5. The gene expression of VEGF, mTOR, PI3K, akt of the gastric cancer cell line SGC-7901 and its correlation of concentration were assayed by RT-PCR.6. The related protein expression of the gastric cancer cell line SGC-7901 influenced by the modified Xuezheng decoction and its correlation of concentration were assayed by Western Blot.Results:1. The modified Xuezheng decoction shows the obvious inhibitory proliferation on the gastric cancer cell line SGC-7901, presenting the evident correlation with time and concentration.2. The modified Xuezheng decoction has obvious anti angiogenesis effect. 3.The modified Xuezheng decoction could significantly induce the apoptosis of the gastric cancer cell line SGC-7901.4. After 48h intervention of different concentration of the modified Xuezheng decoction, the cell cycle of the gastric cancer cell could be blocked at the G2/M phase.5. The modified Xuezheng decoction can down-regulate the mRNA expression of VEGF, mTOR, PI3K, Akt on the gastric cancer cell line SGC-7901.6. Different concentration of the modified Xuezheng decoction can down-regulate the protein expression of VEGFt, mTOR, PI3K, Akt on the gastric cancer cell line SGC-7901.Conclusions:We can conclude that modified Xuezheng decoction can obviously inhibit the SGC-7901 cell proliferation. Modified Xuezheng decoction can induce apoptosis and regulate cell cycle of human gastric cancer cell line SGC-7901. The modified Xuezheng decoction has obvious anti angiogenesis effect. The decoction can regulate the expression of VEGF, mTOR, PI3K, Akt and other metastasis related genes in human gastric cancer cell line SGC-7901, and down regulate the expression of related proteins.