| Objective: Animals of natural brain aging do not satisfy this study of neuroscience,due to the long feeding cycle and high-mortality and significant individual differences.On that basis,this study by subcutaneous injected D-galactose in neck establish brain aging animal model and combined with the method of neurobehavioral analysis and relevant biochemical indexes of serum to evaluated the simulate characteristics of cognitive function of natural brain aging animal.The purpose of this study provided a mature and stable artificial brain aging animal model tool for better serve scientific research.Methods: Sprague-Dawley male rats,aged four months,were randomly divided into three groups(n=40 each): control group,natural brain aging group and D-galactose brain aging model group.The D-galactose brain aging rat were administered subcutaneous 10% D-galactose solution 0.125g/kg for 6 weeks.Rat in the control group received same volume saline for 6 weeks.For the natural brain aging group,rats grew naturally to 20 months.Neurobehavioral manifestation was evaluated by Morris water maze test and Beam-Balance test.SOD activity,CAT and MDA content in serum was measured to evaluating oxidative stress.The weight and food intake of rats were recorded.Results: 1.No different in the weight and food intake among three groups(P>0.05).2.The neurobehavioral test results before establishing model 1)The escape latency of first day was longer than the third,fourth and fifth day of the rats in each group(P<0.05),but there was no statistically significant difference in the escape latency among three groups(P>0.05).2)In the spatial probe test,there was no statistically significant difference among all groups of rats in the residence time,entries,swimming distance and swimming speed of escape platform area,effective platform area,seventh area,third quadrant area and middle-circle area(P>0.05).3.The neurobehavioral tests results contrast analysis before and after establishing model 1)In the spatial probe test,there was statistically significant difference after the D-galactose administration in the residence time,entries and swimming distance of escape platform area,effective platform area,seventh area and third quadrant area(P<0.05).2)In the spatial probe test,there was no statistically significant difference in the swimming speed of rats after the D-galactose intervention(P>0.05).4.The neurobehavioral and oxidative stress specific markers tests results after establishing model 1)The spatial probe test:(1)Compared with the control group,the residence time in the escape platform area,effective platform area,seventh area,third quadrant area and middle-circle area were longer in the natural brain aging group and D-galactose brain aging model group(P<0.05).But no statistically significant difference was found in residence time between the natural brain aging group and D-galactose brain aging model group(P>0.05).(2)No significant difference was found in the entries and swimming distance of the escape platform area,effective platform area,seventh area,third quadrant area and middle-circle area between the natural brain aging group and D-galactose brain aging model group(P>0.05),but both of them groups were lesser than control group(P<0.05).(3)The swimming speed of natural brain aging group was much shorter than control group(P < 0.05).But no statistically significant difference was found in swimming speed between the control group and D-galactose brain aging model group(P>0.05).2)Working memory task: No significant difference was found in the escape latency between the natural brain aging group and D-galactose brain aging model group(P>0.05),but both of them groups were longer than control group(P<0.05).3)Beam-Balance test: No significant difference was found in the start and cross time between the natural brain aging group and D-galactose brain aging model group(P>0.05), but both of them groups were longer than control group(P<0.05).4)Oxidative stress specific markers test: Compared with the control group,MDA content in serum was significantly increased,and the activity of SOD and CAT were significantly decreased in D-galactose brain aging model group(P<0.05),which was similar in the natural brain aging group(P<0.05).This result indicate that D-galactose treatment for 6 weeks could induce ROS production and reduce antioxidant capacity in rats,which was similar to the rats of 20 months.Conclusion: D-galactose induced brain aging model successfully mimics aging process.Therefore,D-galactose induced aging model may be used as an accelerated aging model to study the aged relate neuroscience research... |
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