SGK1/NFAT2 And IL-1? Promote Bladder Smooth Muscle Cell Proliferation And Apoptosis In Bladder Outlet Obstruction

Objective: Bladder outlet obstruction(BOO)is a common urological disease that can cause serious complications.It is very important to study its pathogenesis.We constructed a chronic bladder outlet obstruction model and an acute bladder outlet obstruction model to study the different pathological changes of the bladder between acute and chronic obstruction.Combine the stress-cell proliferation model to reveal the mechanism of disease progression during bladder outlet obstruction.Methods:Female BALB / c mice were divided into three groups: a control group(n = 9),a progressive obstruction group(n = 9),and a direct obstruction group(n = 9).Chronic and acute obstruction models were constructed by gradually narrowing the external urethral orifice and directly narrowing the external urethral orifice.The pathophysiological changes of the bladder were detected 1,2 and 4 weeks after the obstruction.At the same time,different circulating hydrostatic pressure was applied to mouse bladder smooth muscle cells(MBSMC),and then cell proliferation,expression of inflammation and cell cycle were measured.Si RNA interference was used to knock out SGK1 expression in mouse bladder smooth muscle cells,and then NFAT2 expression was detected.Results: Compared with the chronic bladder outlet obstruction model,the acute bladder outlet obstruction model leads to stronger proliferation,inflammation,and fibrosis in the bladder in the early stages of bladder outlet obstruction.Low-level stress stimulation can promote the proliferation of mouse bladder smooth muscle cells.Excessive stress stimulation can inhibit cell proliferation and increase the proportion of mouse bladder smooth muscle cells death.NFAT2 expression was significantly down-regulated when SGKI was knocked out using SGKI si RNA interference experiments.Conclusion:Acute bladder outlet obstruction may lead to faster decompensation of bladder function.Chronic bladder outlet obstruction may be more adaptable than acute bladder outlet obstruction and is a more appropriate disease research model.Appropriate pressure can promote the proliferation of bladder smooth muscle cells,however,greater pressure will reduce the proliferation of bladder smooth muscle cells and promote cell pyrolysis.