Research On The Correlation Between LncRNA-MALAT1 And Inflammatory Cytokines In Peripheral Blood Of Patients With Acute Myocardial Infarction

Objective:The mortality rate of cardiovascular diseases is high among the total causes of death in urban and rural population in China.Long noncoding RNA(LncRNA)is a new type of non-coding RNA,which is not involved in protein coding,but plays an important regulatory role in various biological processes such as proliferation,apoptosis,migration,invasion and differentiation.LncRNA-MALAT1 has been reported to be elevated in plasma of patients with acute myocardial infarction and to be involved in inflammatory processes in other systems as a regulatory factor.This research evaluated the correlation between LncRNA-MALAT1 and IL-6 by measuring the expression level of LncRNA-MALAT1 in peripheral blood of patients with acute myocardial infarction and the level of the inflammatory cytokine IL-6 in plasma,and analyzed the potential of LncRNA-MALAT1 in the treatment of acute myocardial infarction.Methods:40 patients with acute myocardial infarction,40 patients with non-myocardial infarction coronary heart disease,and 40 healthy physical examinees were collected.Serum samples were collected from patients hospitalized in the department of cardiovascular medicine of the second hospital of Jilin university from the October2019 to the January 2020,as well as physical examinees of the same period.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to measure the expression levels of LncRNA-MALAT1 in the three groups of acute myocardial infarction,non-myocardial infarction coronary heart disease and healthy physical examinees.The concentration of IL-6 in the three groups was tested by enzyme-linked immunosorbent assay(Elisa),and the clinical data of the patients were collected.The results were statistically analyzed by spss23.0.Results:1.The level of white blood cell count in the AMI group was(9.59±2.65)×10~9/L,higher than that in the non-myocardial infarction CHD group(7.12±1.55)×10~9/L,and higher than that in the control group(6.77±1.89)×10~9/L,all of which had significant statistical significance(P<0.01).2.The neutrophil count level(7.00±2.50)×10~9/L in the AMI group was higher than that in the non-myocardial infarction coronary heart disease group(4.58±1.15)×10~9/L and the control group(4.20±1.63)×10~9/L,showing significant statistical significance(P<0.01).3.The expression level of LncRNA-MALAT1 in AMI group(8.27±8.44 times)was significantly higher than that in non-myocardial infarction CHD group(0.98±0.73times)and control group(1.00±0.48 times),and all of them had significant statistical significance(P<0.01).4.There was a correlation between the level of LncRNA-MALAT1 and the white blood cell count in the peripheral blood of AMI patients,and the results showed statistical differences(r=0.358,P=0.030,P<0.05).There was a correlation between LncRNA-MALAT1 and neutrophil count levels in peripheral blood of AMI patients,and the results showed statistical differences(r=0.330,P=0.046,P<0.05).5.LncRNA-MALAT1 expression level in peripheral blood of AMI patients was positively correlated with IL-6 level(r=0.373,P=0.021,P<0.05).Conclusion:1.The expression level of LncRNA-MALAT1 in peripheral blood of patients with acute myocardial infarction was significantly increased.2.In this research,the expression level of LncRNA-MALAT1 in peripheral blood of patients with acute myocardial infarction was positively correlated with the level of white blood cell count.The expression level of LncRNA-MALAT1 in peripheral blood of patients with acute myocardial infarction was positively correlated with the level of neutrophil count.3.In this research,the expression level of LncRNA-MALAT1 in peripheral blood of patients with acute myocardial infarction was positively correlated with the expression level of inflammatory cytokine IL-6.