| Objective:Through the study of the intestinal flora between the PD group and the control group,and further analysis of the correlation between the two factors of immune inflammation(Tfh cells,Tfr cells,and CD19 + B cells,as well as IL-4,IL-10,IL-21,and TGF-?)and vitamins(Vitamin B2,3,5,6,12,and 25-hydroxyvitamin D)and significantly different intestinal flora in the PD group.To explore the possible role of immune inflammation and vitamins in the pathogenesis of Parkinson's disease from the "microorganism-gut-brain axis".Methods:Sixteen PD patients who were treated in the Department of Neurology of Bethune First Hospital and 16 matched healthy controls were selected.First,16S-rRNA gene sequencing was used to determine the types of microorganisms in the stool samples,and the richness and diversity of the intestinal flora between the PD group and the control group were analyzed,as well as the differences in the intestinal flora of each taxon.Then the levels of Tfh cells and Tfr cells in peripheral blood of PD group were detected by flow cytometry,and the levels of IL-4,IL-10,IL-21 and TGF-? in serum of PD group were detected by flow cytometry.Then analyze the correlation between the above levels of immune inflammatory cells and factors and intestinal flora.Finally,8 patients were randomly selected from the PD group.The levels of vitamin B groups and 25-hydroxyvitamin D in peripheral blood were detected by tandem mass spectrometry,and the correlation between the above vitamin levels and intestinal flora was analyzed.Results:1.Differences in the distribution of intestinal flora between the PD group and the control group: The richness of the intestinal flora in the PD group was found to be less than that in the control group through the grade abundance curve,but the intestinal flora in the PD group was more uniformly distributed than the control group;there was no statistical difference in the ? diversity index between the PD group and the control group,but the species richness and phylogenetic diversity of the PD group tended to decrease,while the ? diversity tended to increase;through ? diversity analysis,it was found that ? diversity of PD patients was reduced;Lefse analysis and t-test revealed that the relative abundance of 2 classes,2 orders,3 families,10 genera,and 5 species was significantly different between the PD group and the control group.2.Correlation analysis between clinical symptoms and intestinal flora: Acidaminococcaceae was negatively correlated with NMSS,bacilli and lactobacillus were negatively correlated with BMI.3.Correlation analysis of immune inflammation and intestinal flora: Spearman correlation analysis was performed on the levels of Tfh cells,Tfr cells,B cells,IL-4,IL-10,IL-21 and TGF-? in the peripheral blood of the PD group and intestinal flora.There were significant differences in the correlation between intestinal flora and inflammatory cells and factors.Among them,Negativicutes,selenomonadales,and Veillonellaceae were negatively correlated with Tfh(P <0.05);Veillonellaceae was correlated with IL4(P <0.05)and IL10(P < 0.05)was negatively correlated;Megamonas and Phascolarctobacterium were positively correlated with CD19 B cells(P <0.05).4.Correlation analysis of vitamins and intestinal flora: Spearman correlation analysis was performed on the levels of VitB2,B3,B5,B6,B12,and 25-hydroxy VitD in the peripheral blood of the PD group with the intestinal flora.The main focus was on the intestinal flora with significant differences in the T-test test.The correlation of vitamins was found to have a negative correlation between Veillonellaceae and VitB2(P <0.01);a positive correlation between Roseburia and Roseburia-inulinivorans and VitB5(P <0.05);Clostridium_sp_L2-50 species and VitB3(P <0.05)was negatively correlated.Conclusion:1.The relative abundances of 2 classes,2 orders,3 families,10 genera and 5 species of intestinal flora were significantly different between the PD group and the control group.2.NMSS and BMI are related to the abundance of PD intestinal flora,suggesting that microorganisms may participate in the regulation of clinical symptoms.3.Tfh cells,CD19+B cells,IL4 and IL10 are related to the abundance of PD intestinal flora,suggesting that immune cells and inflammatory factors are involved in the regulation of microorganisms.4.VitB2,VitB3 and VitB5 are related to the abundance of PD intestinal flora,suggesting that vitamin and metabolite levels may be involved in the regulation of microorganisms. |
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