ZnDPA-Conjugated Cyanine Dyes For Apoptosis And Inflammation Targeted Detection

Apoptosis is involved in the operation of the human immune system.The production of high-risk diseases such as inflammation,Alzheimer's disease,cancer,and cardiovascular and cerebrovascular diseases is inextricably linked to apoptosis.Therefore,it is necessary to develop apoptotic probes with high targeting and low cytotoxicity.It is well known that valgus phosphatidylserine(PS)is one of the common apoptosis targets.The large molecular protein Annexin V,a commercial apoptosis kit,has good targeting,but its preparation process is complex and expensive.And chemically unstable.Based on the chemical structural characteristics of eversion PS,this paper explored the synthesis of ZnDPA-Cy cyanine dyes(ZnDPA-Cy)with different emission wavelengths that have high targeting for eversion PS.By analyzing the structural and optical properties of the materials,they were applied to three human cancer cells by means of flow cytometry,laser confocal and photoacoustic methods.In the detection of apoptosis,the differences of PS targeting effect between this kind of material and the commercial macromolecular protein Annexin V were explored by double staining.In addition,based on the close relationship between inflammation and apoptosis,this topic explored the targeting of materials to mouse liver injury models.ZnDPA-Cy7 was selected as the targeted investigative material according to the characteristics of the materials and instruments,using photoacoustic methods,Completed the photoacoustic and ex vivo photoacoustic experiments of the materials on mice with acute liver injury,and analyzed the experimental results,which laid the foundation for the targeted detection of apoptosis-related diseases in the materials.