Endoplasmic Reticulum Stress In The Early-stage Of Diabetic Skin Lesion

Diabetic skin lesion is one of the most common diabetic complications and is the pathological foundation of the diabetic chronic wounds.Endoplasmic reticulum stress has been observed in diabetes mellitus and some of its complications.However,further study is needed to explain the accurate mechanism and its role in the diabetic skin lesion.Objective:To observe the pathological change of diabetic skin at different time stage.To investigate whether endoplasmic reticulum stress exists in the diabetic skin lesion and explore its role at the early stage.Materials and methods:C57BLKS db/db mice were studied at the age of 6,9 and 12 weeks.Each group was consisted of 6 db/db mice and 6 db/m mice as control.The basic pathological change of the diabetic skin were observed.Protein and gene expression of endoplasmic reticulum stress were analysed at the same time.The apoptosis of skin tissue was detected by TUNEL.Results:the pathological change in the db/db mice skin tissue become evident at the age of 9 weeks and even more prominent at 12 weeks.Thickness of skin tissue kept decreasing and the formation of collagen was abnormal.Expression of protein and gene of endoplasmic reticulum stress increased in db/db mice from 6 weeks.There was no significant apoptosis in the skin tissue until 9 weeks,and the apoptosis became prominent in the db/db mice at 12 weeks.Conclusions:Skin lesion occurs at the very early stage of diabetic mellitus.The endoplasmic reticulum stress exists in the diabetic skin tissue and progresses with time.The apoptosis gene of the endoplasmic reticulum stress overexpressed in the diabetic mouse than in the control.No obvious apoptosis was observed in the diabetic skin tissue at the early stage before the endoplasmic reticulum stress was activated...