Construction Of Specific Biosensors Against Monocyte Chemotactic Protein-1

Atherosclerosis is the most important cause of cardiovascular disease.Among them,mononuclear chemotactic protein-1?MCP-1?has a great relationship with the pathogenesis of cardiovascular disease,and the level of human serum MCP-1 is increased.It can cause atherosclerotic diseases such as unstable angina pectoris,myocardial infarction,and in-stent restenosis.Therefore,the determination of MCP-1 in serum is of great significance for the diagnosis and prevention of atherosclerotic diseases.Although the traditional detection method is accurate,sensitive,and effective,it has the disadvantages of being time-consuming,expensive,and relatively complicated.In recent years,electrochemical sensors have provided a new research idea for the detection of MCP-1 due to its high sensitivity,good stability,simple operation and low cost.This article focuses on two aspects of how to prepare suitable nanomaterials,how to choose the optimal detection conditions,and how to analyze the analytical performance to construct a new type of biosensor.The research work is mainly divided into the following two parts:1.Construction of ultra-sensitive sandwich immunochemical sensors based on reduced graphene oxide-thionine-gold nanocomposites?rGO-TEPA-Thi-Au?and new ruthenium-palladium-platinum trimetal nanoparticles?RuPdPt TNPs?for MCP-1 detection researchIn this study,thionine molecules?Thi?are adsorbed on the surface of reducing graphene oxide-tetraethylenepentamine?rGO-TEPA?due to their own charge characteristics and?-?interactions,while AuCl₄^-is a gold nanoparticle formed by reduction The particles?AuNPs?are stabilized by Thi and combined with rGO-TEPA through amino groups.In addition,AuNPs in the nanocomposite can provide a large number of active sites for immobilized biomaterials to immobilize the first antibody of monocyte chemotactic protein-1?MCP-1-Ab₁?.In addition,another nanomaterial RuPdPt TNPs involved in this experiment has excellent performance in catalytic reduction of H₂O₂,and the second antibody of mononuclear chemokine-1?MCP-1-Ab₂?can be efficiently captured by Pt-NH₂.We use These two features use RuPdPt TNPs as the signal response part of the sensor.Based on the above-developed sensors,MCP-1 can be efficiently identified in serum by chronoamperometry with a detection range of 0.02-10³ pg mL^-11 and a minimum detection limit of 8.9 fg mL^-1.The sensor not only has a wide linear range and low detection limit,but also has great prospects in early clinical diagnosis and guidance of medication.2.Study on the detection of MCP-1 by an internal reference electrochemical luminescence sensor based on sulfur-doped palladium nanosheets?s-PdNS?In this study,electrochemical luminescence?ECL?has been widely used for the detection of serum markers due to its high sensitivity,simple operation,and fast response speed.However,any previous detection method may be due to instrument or environmental factors.The change produces uncontrollable results.In order to improve the accuracy of detection,we synthesized a new nanomaterial s-PdNS and modified the traditional luminous compound Luminol on the nanomaterial by using the characteristics of the material.On the one hand,Luminol modified s-PdNS acts as the acceptor of the cathode S₂O₈^2-/O₂ system to quench the cathode ECL signal.On the other hand,modified Luminol introduces a new anode ECL signal.When the cyclic voltammetry scans different potentials,two different signals will appear,which not only eliminates the mutual interference between the signals but also successfully avoids possible detection errors.In addition,AuNPs deposited directly on the electrode surface not only stably provided the active sites of fixed biomolecules,but also greatly increased the electron transfer rate and made the changes in ECL signals more pronounced.Due to its simpler construction,the electrochemical luminescence sensor has a wider detection range compared to previous research,10^-2.5-10³ pg mL^-1,and a lower detection limit of 1.6fg mL^-1,excellent specificity and Repeatability also provides a new idea for clinical research.Based on the above studies,we further verified the guiding role of the sensor for clinical use in cell experiments.By comparing rosuvastatin calcium to lipopolysaccharide?LPS?-induced human THP-1 monocyte monocyte chemotactic protein-1?MCP-1?expression of ELISA detection and sensor detection results,we know that our biosensors have great application potential in guiding drug use.