Comparison Of IL-1β In Rats With Traumatic Brain Injury At Different Altitudes

Objective: In this study,the contents of interleukin-1β in the injured brain tissues of the rats with traumatic brain injury at different altitudes of 4500 meters(the plateau area)and 2200 meters(Xining area)were compared to investigate the effect of different altitudes on the expression of interleukin-1β in the injured brain tissues of the rats with traumatic brain injury,so as to guide the prognosis evaluation and clinical treatment of patients with traumatic brain injury in areas at different altitudes,especially in the plateau area.Methods: A total of 60 healthy Sprague-Dawley male rats were randomly and equally divided into the plateau area group and Xining area group.25 rats in each group were randomly selected for free fall after dural exposure to establish the rat model of traumatic brain injury(the model group).The remaining 5 rats in each group were exposed to dural mater only and did not receive free fall(the control group).On the 1st,3rd,5th,7th and 14 th day after traumatic brain injury,the enzyme-linked immunosorbent assay was used to test the contents of interleukin-1β protein in the injured brain tissues of 5 rats from each group.And the brain tissues of the rats from the control groups were sampled and tested at the same time.SPSS 22.0 software was used to analyze the data,with t-test used for the comparison between the contents of interleukin-1β from diffrent altitudes and one-way analysis of variance used for the comparison of the contents of interleukin-1β between different time points at the same altitude.Results: All the contents of interleukin-1β in the injured brain tissues of the model rats in both the plateau area and Xining area were higher than that in the control groups,and besides the difference between contents of interleukin-1β in the injured brain tissues of the model rats in Xining area on the 14 th day and that of the control group at the same time was not statistically significant(P>0.05),all differences were statistically significant(P < 0.05).The contents of interleukin-1β protein in the injured brain tissues of the model rats in both the plateau area and Xining area after traumatic brain injury increased significantly on the 1st day,peaking on the 3rd day,maintaining a relatively high level on the 3 ~ 5th day,significantly decreasing on the 7th day,continuing down to the 14 th day.At all time points after traumatic brain injury,the contents of interleukin-1β in the injured brain tissues of the model rats in the plateau area were significantly higher than that in the model group in Xining area,with differences being statistically significant(P < 0.05).On the 14 th day,the contents of interleukin-1β in the injured brain tissues of the model rats in the plateau area were higher than that in the control group,with the difference being statistically significant(P < 0.05).The contents of interleukin-1β in the injured brain tissues of the model rats on the 14 th day in Xining area was also higher than that in the control group,but with the difference being not statistically significant(P > 0.05).Conclusion: Altitudes can affect the content of interleukin-1β: the higher the altitude,the higher the content of interleukin-1β in the injured brain tissues of rats after traumatic brain injury,and the slower its fading.