Application Of BSP In Detecting TGFBI Gene Methylation In Han And Tibetan Gastric Cancer Patients In QingHai Province

Objective: To investigate whether TGFBI gene methylation is related to the occurrence and progress of gastric cancer(GC)by detecting the methylation status of TGFBI gene promoter in GC and its matched adjacent mucosa.And to explore the difference in the methylation of TGFBI gene promoter between Han and Tibetan patients with GC to provide theoretical datas for the high incidence of GC in Qinghai and the possible causes of the different incidence between Han and Tibetan.Methods:1.28 cases of gastric mucosa biopsies were collected which from GC patients who were diagnosed by gastroscopy and gastric mucosa biopsy in the Affiliated Hospital of Qing Hai University from March 2019 to November 2019.Among them,there were 28 cases of gastric mucosa and 28 cases of its matched adjacent mucosa which in its paired non-gastric cancer mucosa.2.According to different ethnic groups,there are 17 Han and 11 Tibetan.3.The methylation of TGFBI gene was detected by bisulfite sequencing PCR(BSP).Result:1.The methylation degree of TGFBI gene promoter showed that there were 478 methylated clones in GC group,and average methylation rate was 11.42%;there were 293 methylated clones in matched adjacent mucosa group,and average methylation rate was 6.98%;The methylation degree of TGFBI gene promoter was significantly higher in GC group than matched adjacent mucosa group(P<0.05).2.In the GC groups of Han(17 cases)and Tibetan(11 cases),Han showed 299 methylation clones with an average methylation rate of 17.73%;Tibetan showed 179 methylation clones with an average methylation rate of 10.95%.There was no significant difference in the methylation of TGFBI gene promoter between Han and Tibetan GC groups(P > 0.05).3.In the matched adjacent mucosa groups of Han(17 cases)and Tibetan(11 cases),Han showed 208 methylation clones with an average methylation rate of 8.16%;Tibetan showed 85 methylation clones with an average methylation rate of 5.15%.The methylation degree of TGFBI gene promoter was significantly higher in Han than Tibetan in matched adjacent mucosa groups(P<0.05).4.Analysis of the collected clinicopathological data showed that there was no significant difference in the methylation degree of TGFBI gene promoter of GC,compared with the gender,age,pathological differentiation,depth of invasion,complications of the other tissues and organs and TNM stage(P > 0.05).Conclusion:1.The methylation degree of TGFBI gene promoter in GC group was significantly higher than its matched adjacent mucosa group.It showed that the higher hypermethylation of TGFBI gene promoter may be involved in the occurrence and development of GC.2.The methylation of TGFBI gene promoter was significantly higher in Han than Tibetan GC matched adjacent mucosa groups,but there was no significant difference in the methylation degree of TGFBI gene promoter in Han and Tibetan GC groups,this result may provides reference for the research direction of early GC diagnosis and the effect of gene methylation on the incidence of different ethnics with GC.3.There is no statistical significance between the methylation of TGFBI gene promoter in GC and complications of the organs or TNM stage.