Minocyclinie-loaded PLGA Electrospun Membrane Prevents Alveolar Bone Loss In Experimental Peridontitis

Chronic periodontitis is one of the most common oral diseases.The continuous alveolar bone loss(ABL)caused by this condition leads to teeth loosening and,eventually,teeth loss.Periodontitis is the main cause of missing teeth in adults.Traditional periodontal treatment can control periodontal inflammation effectively,but the reconstruction of alveolar bone resorption caused by periodontitis is still a huge challenge.Minocycline(MINO)is a tetracycline antibiotic effective against most of the bacteria microorganisms related to periodontal disease.Additionally,MINO promotes bone in vitro and in vivo.However,the high concentration of minocycline in the periodontal pocket will inhibit the proliferation and differentiation of osteoblasts.In order to avoid excessive local antibiotic concentration,the effectiveness of the submicrobial dose of minocycline and its local administration have become the focus of recent research.Electrostatic spinning technology can obtain a variety of stents with different characteristics through the principle of electrostatics,which are used for drug delivery and tissue regeneration.Electrospinning technology is widely used because of its advantages like extracellular matrix,high load capacity,high packaging efficiency,easy operation,low cost and high efficiency.In this study,polylactic acid-glycolic acid(PLGA)electrospun membranes loaded with different concentrations of minocycline were prepared.Their release and degradation properties in vitro were studied.The optimal concentration of MINO-PLGA electrospun membranes was selected for in vivo experiments.MINO-PLGA electrospun membranes' s effect on alveolar bone reconstruction of experimental periodontitis in rats was studied.Method:1.The amount of MINO released from the membrane was measured by absorbance at 375 nm,the optical density(OD)values were converted to concentrations using a standard curve.The cumulative release curve of minocycline was fitted to the mathematical model.The weight of the MINO-PLGA electrospun membrane at different time points was measured,and the cumulative weightlessness curve of the electrospun membrane was ploted.2.SD rat experimental periodontitis model was eatablished by orthodontic steel wire ligation and 10% high sugar water feeding for 4weeks.3D images were reconstructed and the alveolar bone loss ABL was calculated through micro-CT.HE staining and immunizationhistochemical staining(osteoprotegerin(OPG),the ligand of the receptor activator for nuclear factor-?B(RANKL))were performed.Result:The drug release study showed a sustained diffusion of MINO from electrospun membranes over a period of 40 days.The remaining weight of the electrospun membrane was approximately 52% at 60 days.MINO-PLGA membrane increased alveolar crest height in the periodontitis model,inhibited the expression of the ligand of the receptor activator for nuclear factor-?B(RANKL),and promoted the expression of its inhibitor,osteoprotegerin.The study demonstrated that MINO-PLGA electrospun membranes may be applied to stimulate bone regeneration in periodontitis.The above results indicate that the MINO-PLGA electrospun membrane has good slow release and degradation properties,and in vivo experiments have also confirmed that the MINO-PLGA electrospun membrane can promote the reconstruction of periodontal bone tissue.Therefore,the MINO-PLGA electrospun membrane is expected to be used as a one-time controlled release system for the treatment of periodontitis bone defects.