Infiltration Patterns Of Immune Cells In Cutaneous Melanoma And Its Correlation With Prognosis

Objective : To explore the characteristics of immune cell infiltration in cutaneous melanoma and the relationship between immune cell infiltration and prognosis.Method : Download the gene transcription data and clinical data about cutaneous melanoma in The Cancer Genome Atlas(TCGA).Use Cibersort software to calculate the relative proportion of 22 kinds of immune cells in melanoma.Use R programming language to calculate the correlation between the proportion of immune cells and gender.Use Kaplan-Meier survival analysis method to calculate the correlation between the overall survival time of patients and the proportion of 22 immune cells.Results : A total of 472 cases of gene transcription data(470 cases of melanoma tissue and 2 cases of normal skin tissue)and 470 cases of melanoma clinicopathological data were downloaded.Use Cibersort calculation software to convert the above-mentioned gene transcription data of each sample to the proportion of each immune cell in the sample.219 cases of cutaneous melanoma and1 case of normal skin tissue were obtained by screening samples with P?0.05.CD8+ T cells,M0 macrophages,and M2 macrophages are the types of immune cells with higher levels of infiltration in melanoma tissues.The strongly correlated immune cells include resting memory CD4+ T cells and CD8+ T cells(-0.53),CD8+ T cells and M0 macrophages(-0.62),neutrophils and activated mast cells(+0.7).Resting mast cells,resting NK cells,and ??-T cells can serve as independent prognostic factors for patients.Conclusion :There are differences in the composition of infiltrating immune cells in cutaneous melanoma,and different infiltration patterns may affect the prognosis of patients,which helps us develop effective immunotherapy.