Chemical Constituents Of The Curculigo Capitulata From Hainan

Curculigo capitulata is a perennial herbaceous plant of the genus(Curculigo)of the family(Hypoxidaceae).It is a traditional Chinese medicine with diuretic,anti-inflammatory and analgesic effects.Various types of compounds have been isolated and identified from this genus.Including;norneoligans and their glycosides,phenoloids and their glycosides,alkaloids,flavonoids,volatile oils and trace elements.It has been found that the genus has pharmacological effects such as antidepressant,regulating immunity,tonifying kidney and strengthening yang,anti-osteoporosis,anti-tumor,anti-inflammation and antifungal,protection of liver and so on.There are more than 20 species of Curculigo in the world,and 7 species are mainly distributed in South China and southwest China,but there are no literature reports on the production of C.capitulata in Hainan.On this basis,We carried out the study on the chemical constituents of C.capitulata produced in HainanObjective:To isolate,purify and identify the chemical components from C capitulata.To expolre he difference of Chemical constituents between Hainan and other producing areas of C.capitulata.Methods:In this study,95%ethanol extract was separated and purified by using macroporous resin and MCI,ODS,C18,to prepare silicone thin layer plate,silica gel column,HPLC and other chromatographic techniques,and then nuclear magnetic resonance(NMR)was used.The structure of the isolated monomer compounds was identified by organic spectral analysis such as mass spectrometryResults:26 compounds were isolated from C.capitulata produced in Hainan,and 24 compounds were identified.The results were as follows:2,6-dimethoxybenzoic acid(1),2,6-dihydroxytoluene(2),2,6-dimethoxypyridine(3),P-Hydroxybenzoic acid(4),Gallic acid(5),Shikimic acid(6),Methylshikimate(7),Physcion(8),(S)-12,14-dihydroxy-3-methyl-3,4,5,6,7,8,9,-10-octahydro-lH-benzo[c][1]oxacyclododecin-1-One(9),Orcinol glucoside(10),Dimethyl phthalate(11),P-hydroxybenzoyl P-coumaricadanhydride(12),?-sitostenone(13),?-Sitosterol Acetate(14),Sitosterol(15),Daucosterol(16),Uridine(17),3-hydroxy-5-methlphenol-1-O-?-D-glucoPyran-osyl-(1?6)-?-D-Glucop-yranoside(18),Typhonosid-e C(19),3-hydroxy-2-oxopentac-osanoic acid(20),3,5-dihydroxy-2-carbonyl heptadecanoic acid(21),Methyltetr-acos-anoate(22),Palmitic acid(23),(n)-tetracosanoic acid(24)Conclusion:14 compounds of 3,4,5,6,7,9,12,13,14,19,20,21,22 were found for the first time in the genus(Curculigo);At present,it has been found from the identified compounds that there are fewer norneolignans compounds and more small molecular phenolic compounds(2,4,5,8,9,10,11,12,18)in C.capitulata from Hainan.The difference of secondary metabolites of the same plant is caused by the difference of secondary metabolites of the same plant,which may be due to the different soil environment,climatic conditions and altitude and so on.