Effect Of Warm Acupuncture "Jiaji" Acupoint On The Expression Of OX42,GFAP And P38MAPK In Spinal Cord Of CCI Model Rats

Objective:By observing the Warm acupuncture�jiaji�point different time points after the sciatic Chronic constriction injury model?CCI?in the rat spinal cord waist promote silk crack enzyme original P38 Mitogen-activated protein lightning?P38MAPK?,astrocytes markers Colloidal fibrillar Acidic protein?GFAP?and clonal name of monoclonal antibody of complement receptor type 3?OX42?expression in spinal microglia cells were used to investigate the relationship between warm acupuncture analgesia and activation state of microglia cells,so as to further reveal the mechanism of warm acupuncture analgesia and provide theoretical basis for clinical application.Methods:Clean-adult male SD?Sprague Dawley?rats were adaptively fed for 7 days,and then screened for heat pain threshold.72 rats were selected and randomly divided into 12 blank groups,12 model groups and There are 48 warm acupuncture groups.The warm acupuncture group was divided into 4 subgroups immediately after warm acupuncture,1h,2h,and 12h according to different time points after warm acupuncture,each group had 12 rats.Six rats were randomly selected from each group,and there was no significant difference in the threshold of heat pain?P>0.05?.Both the model group and the warm acupuncture group established a chronic sciatic nerve compression injury?CCI?model,and the blank group was left untreated.On the 7th day after the warm acupuncture group,take bilateral L455 Jiaji points to pierce the acupuncture approximately 23mm,and require the acupuncture tip to reach the lamina.The L4 and L5�Jiaji�points on each side should be of the same size as soybeans.The conical moxa is inserted into the acupuncture handle and ignited.The warm acupuncture part of the rat's back is insulated with a gasket.The temperature is based on the tolerance of the rat.The acupuncture retention time is 20 minutes.Each group of rats receives intervention once.The rats in the model group and the blank group received fixed intervention only.Six rats in each group were randomly selected on the 7th day?pre-intervention?after modeling,and the heat pain threshold was measured before the material was taken.Each warm acupuncture group was taken immediately,1h,2h,and 12h after the warm acupuncture intervention.The model group was selected immediately after the fixed intervention.According to the random number table method,6 rats in each group were selected according to the requirements of Western Blot,and the other 6 were selected according to the requirements of immunohistochemistry.Immunohistochemical technique was used to detect the expression of GFAP,P38MAPK and OX42 proteins in the spinal dorsal horn?SDH?of the right spinal cord lumbar enlargement of rats.expression.Results:?1?After 7 days of modeling,compared with the blank group,the thermal pain threshold of the warm acupuncture group and the model group significantly decreased?P<0.01?,and hyperalgesia appeared;?2?After the warm acupuncture intervention,compared with the model group,The heat pain threshold was significantly increased in the 1h,2h and blank groups?P<0.01?,and the heat pain threshold was not significantly increased in the 12h group after warming acupunctures?P>0.05?.?3?The Western Blot results showed that compared with the model group,the heat pain immediately after warming acupunctures The GFAP protein was significantly down-regulated in the 1,2,and 2h groups and the blank group?among them,P<0.01 in the 1h group immediately after warming acupunctures,P<0.05 in the 2h group after warming acupunctures?;the GFAP protein was not significantly reduced in the 12h group after warming acupunctures?P>0.05?;Compared with the model group,P38MAPK protein was significantly down-regulated immediately after warming,1h group and blank group?P<0.01?;P38MAPK protein was not significantly down-regulated at 2h and 12h after warming acupuncture?P>0.05?.?4?The results of immunohistochemistry showed that compared with the model group,GFAP protein was significantly down-regulated immediately after warming,1h group and blank group?P<0.01?;GFAP protein was not significantly down-regulated at 2h and 12h after warming acupuncture?P>0.05?.Compared with the model group,P38MAPK protein was significantly down-regulated immediately after warm acupuncture,1h,2h group and blank group?among them,P<0.01 immediately after warm acupuncture,2h group,P<0.05 after warm acupuncture 1h group?;P38MAPK in 12h group after warm acupuncture The protein down-regulation was not significant?P>0.05?;compared with the model group,the OX42 protein was significantly down-regulated immediately after warming acupuncture,1h group and blank group?P<0.01?;the OX42 protein was not significantly down-regulated at 2h and 12h after warming acupuncture?P>0.05?.Conclusion:1.After modeling,the CCI model rats showed hyperalgesia.Warm acupuncture at�Jiaji�points can significantly increase the thermal pain threshold of the CCI model rats and have an analgesic effect on pathological pain rats.2.The mechanism of warming the analgesic effect may be to increase the heat pain of CCI model rats by inhibiting the activation of glial cells in the dorsal horn of the spinal cord and down-regulating the expression of GFAP and OX42 related proteins in the spinal cord and inhibiting the activation of the P38MAPK signaling pathway Threshold.3.The persistent effect of warming acupuncture analgesia can be extended to 2 hours or more after warming acupuncture.