| Insulin-like Growth Factor Binding Protein 2(IGFBP-2)is a member of Insulin-like Growth Factor Binding Protein(IGFBPs)family,which is highly conserved and involved in a variety of complicated life processes.IGFBP-2 can bind with Insulin-like Growth Factor(IGF)and locally regulate the concentration of IGF.It can also independently exert biological functions.It is found in a research that IGFBP-2 levels were significantly higher in the offsprings of long-living people in bama,guangxi than in those of non-longliving people.Neurogenesis has been found to happen in long-living mice.And It is found in another study that IGFBP-2 levels in plasma and cerebrospinal fluid were higher in alzheimer’s disease patients than in normal elderly individuals,suggesting a possible link between these phenomena.Neural stem cells are pleiotrophic and are able to differentiate into neurons or glial cells,release nutrient factors,replace damaged neurons,as well as maintain a homeostasis of the amount of synapses.Highly rated,neural stem cell transplantation is a new treatment for alzheimer’s disease.Exploring the effects of IGFBP-2 on the life activities of neural stem cells and the underlying mechanism may be helpful to better understand the role and mechanism of neural stem cell transplantation in alzheimer’s disease,to decipher the unknown role of IGFBP-2 in brain development and brain function,and to further reveal the secret of high expression of IGFBP-2 in the offspring of long-living people.To explore the effects of IGFBP-2 on the life activities of neural stem cells,this study first applied real-time unlabeled,CCK8 and scratch test to primarily inquire into the proliferation,cell activity and cell migration of C17.2 cells treated with IGFBP-2.As is shown in the results,IGFBP-2 inhibites the proliferation of C17.2 cells,decreases the cell activity,and significantly inhibites the migration of C17.2 cells at high concentration.Then,this study used the iTRAQ proteomics technology to qualitatively and quantitatively determine differential proteins between C17.2 neural stem cells processed by IGFBP-2 and the blank control group.Information of differential proteins were then imported into the GO database,KEGG database,StringDB protein interaction database.In terms of comprehensive differences in protein related biological processes,cell composition,molecular function,main enrichment of signaling pathways,and protein interaction network information,it is concluded that phosphatidic inositol-3-kinase is the core protein in KEGG enrichment signaling pathway and protein interaction network and PI3K-AKT-mTOR signaling pathway is the core signaling pathway.All the aforementioned data indicate that the existence of IGFBP-2 may be detrimental to the growth and development of neural stem cells. |
