The ITRAQ Technology Was Used To Identify Differentially Expressed Protein Molecules In Different States Of Bone Marrow Niche

In the treatment of leukemia,chemotherapy combined with hematopoietic stem cell transplantation is still the main strategy.However,in the human body,hematopoietic stem cells is extremely rare.Before bone marrow transplantation,bone marrow matching is necessary but the probability of success is relatively low.So clinically,it is often not possible to get enough hematopoietic stem cells.In vitro expansion of hematopoietic stem cells is one of the possible ways to solve these problems,by adding a variety of related factors into culture system to regulate the quiescence,differentiation and proliferation of hematopoietic stem cells,while maintaining its ability to self-renewal and hematopoietic reconstruction.People attempted to use these factors(or drugs)alone or in combination to promote the in vitro expansion of hematopoietic stem cells,but have not yet made a determinate breakthrough.The practicability of in vitro expansion is still very limited,and the reason may be some of the key factors have not yet been found.In this study,we used iTRAQ mass spectrometry and bioinformatics tools to analyze the changes in protein composition in the extracellular fluid of mouse bone marrow during hematopoietic recovery(during which hematopoietic stem cells were amplified)and attempted to screen related proteins.We found 117 proteins which we thought related to HSC expansion from about 1200 proteins in total.We hope this research provide valuable information for hematopoietic stem cells in vitro amplification-related research in the future.