Effect Of Functional Position External Fixation On The Expression Of NF-?B Pathway Related Genes In Rabbits After Achilles Tendon Injury

Objective A functional plaster fixation model after repairing acute injury of Achilles tendon in rabbits were established.To investigate the effect of functional external fixation on the expression of nuclear transcription factor-?B(NF-?B)pathway-related genes in rabbits after Achilles tendon injury.Methods Adult New Zealand white rabbits with specific pathogen free(SPF)undergone an "Acute Achilles Tendon Rupture Modeling + Achilles Tendon Rupture Repair Surgery" on the right lower limb.After rupture of the Achilles tendon,functional gypsum external fixation(group A)and ankle joint extension gypsum external fixation(group B)were repaired,and the sham group(group C)was set up with only skin incision and no Achilles tendon injury.Achilles tendon tissue and peripheral blood were taken1,3,5,7,14,28 days(days,d)after repair of tendon rupture,and grade the adhesion degree of the Achilles tendon according to the adhesion score standard,and count the number of animals in each grade.RT-PCR method was used to detect the inhibitor of NF-?B kinase alpha(IKK?)and NF-?B P65 m RNA in NF-?B inhibitor(I?B)in tendon tissue.phosphate dehydrogenase,GAPDH)as an internal reference gene.The enzyme-linked immunosorbent assay(ELISA)method was used to detect the level of NF-?B in animal serum.Statistical analysis was performed on the data obtained using SPSS13.0.Student-t test was used to compare data between groups.Repeated measures analysis of variance was used to compare data between different time points within the group.The test level ? = 0.05,P <0.05 was considered statistically significant.Result New Zealand white rabbits in each group did not die from the end of the operation to the end of the experiment;the wounds had good healing effects,and all animals showed no wound infection.The gypsum used in the experiment was tough and firm,with no abnormalities.The number of animals with Grade 1Achilles tendon adhesion in Group A was 10%,Grade 2 with 80%,Grade 3 with 5%,Grade 4with 5%,and Grade 5 with 0%.Group B were Grade 2 with 20%,Grade 3 with 50%,Grade 4with 30% and Grade 5 with 0%.Compared with group B,the adhesion level of group A wassignificantly lower than that of group B.RT-PCR results: From 1 d to 28 d,IKK?(P = 0.042,P = 0.020)and NF-?B P65 m RNA(P = 0.039,P = 0.018)in the Achilles tendon tissues of Group A and Group B over time Increase and increase.From 3 d to 28 d,compared with group B,the levels of IKK? and NF-?B P65 m RNA in group A were lower than those in group B(both P <0.05).ELISA results: Consistent with RT-PCR results,from 1 d to 28 d,serum NF-?B levels in groups A and B increased with time(P = 0.033,P = 0.014).From 3 d to 28 d,compared with group B,the level of NF-?B in group A was lower than that in group B(both P <0.05).NF-?B levels in group A returned to group C levels at 28 d.Conclusion(1)The NF-?B in serum of experimental rabbits after acute injury repair of Achilles tendon decreases with the time of repair operation;(2)The transcription level of NF-?B P65 and IKK? gradually decreases with the time of repair operation;Achilles tendon adhesion after external fixation on the injured Achilles tendon is lower than that of traditional straight external fixation;(4)Transcription level of NF-?B P65 and IKK? and serum NF-?B level in the group of gypsum functional external fixation Lower than the traditional straight external fixation group.