| Objective Based on the theory of Mongolian medicine,this subject takes a rat model of chronic renal failure as the research object,observes the general condition of rats,the effects of BUN and SCr on renal function and the pathological and morphological changes of renal tissue,and discusses Mongolian medicine to Gexiguna-3 pharmacological effects of soup.Methods Eighty SPF-grade healthy male SD rats weighed 170-220 g.Eighty were randomly divided into five groups: blank control group(K),sham operation group(J),model group(M),Gexiguna-3 soup group(GZ),and positive control group(Y).1.Establish 5/6 nephrectomy rat model of chronic renal failure.2.Observe the general state,weight and diet of rats in each group after modeling.The rats were put into metabolic cages one day before material collection,urine was collected and urine volume was recorded,and urine routine,urine creatinine(Ucr)and total 24-hour urine protein(UTP)were measured with a urine analyzer.After the last administration,blood was taken from the abdominal aorta,and the serum was separated and the three items of renal function(BUN,Scr,UA,BUN / Scr)were measured with a biochemical analyzer;six serum ion(Na,K,Ca,P,Mg,Cl)and other indicators.3.Enzyme standard method was used to detect plasma SOD activity,SOD,MDA.4.Take the left kidney of each group of rats,use HE staining to observe the pathological changes of kidney tissue under light microscope.Results Experiment one:(1)General conditions: The control group rats in the blank group were always agile,responsive,body hair was dense and smooth,neat and shiny,auricles,lips,eye and nose mucosa were ruddy,and eating and drinking were normal.The general situation of the sham operation group was basically the same as that of the blank group.The surgical scar of about 2.5 cm in length in the bilateral renal region of the dorsal costal spine angle was faintly visible.Model group: thin body,dull eyes,dull reaction,debilitating mental state,dull and sparse body hair,obvious pale or purple ears and paws,thin stools,small amount of urine,and even hematuria.(2)Body weight: The rats were kept adaptively for one week after purchase.The body weight of each group of rats was 200-300 g before modeling,with no significant difference(P> 0.05).After 4 weeks after modeling,there was no significant difference in body weight between the groups(P>0.05).However,the weight of the model group tended to decrease after surgical modeling.After 8 weeks,12 weeks,16 weeks and 20 weeks of modeling,the body weight of the model group was significantly lower than that of the blank control group.(3)Blood routine: Compared with the blank control group,there was no significant difference in the WBC,RBC,PLT count and HGB content in the blood of the model group rats(P>0.05).Compared with the model group,there was no significant difference in the content of WBC,RBC and HGB in the blood of the rats in the drug administration group(P>0.05);there was a significant difference in the content of PLT in the blood of the rats in the medication group(P<0.05).(4)Urine routine: Compared with the empty control group,there was no significant difference in Ucr between the model group and the Gexiguna-3 soup group and the positive control group(P>0.05).Compared with the model group,there was no significant difference in Ucr between Gexiguna-3 soup group and positive control group(P>0.05).There was no significant difference between the Gexiguna-3 soup group and the positive control group(P>0.05).Experiment two:(1)Renal function: Compared with the blank control group,urea nitrogen and blood creatinine,the model group was significantly increased,the difference was significant(P<0.05),indicating successful modeling;compared with the model group,the urea nitrogen There was no significant difference in blood creatinine(P> 0.05);there was no significant difference between the Gexiguna-3 soup and the positive control group(P>0.05).(2)Electrolyte: Compared with the empty control group,the levels of electrolyte indicators Na,Ca,K,Cl,P,and Mg in the blood of the model group were not statistically different(P>0.05).Compared with the model group,the blood levels of K,Ca,and Mg in the positive control group were statistically different(P<0.05),and other electrolyte indicators such as Cl and P levels were significantly different(P <0.01);There was a statistically significant difference in the level of P in the blood of rats in Gexiguna-3 decoction group(P<0.01).It indicates that there are symptoms of electrolyte metabolism disorder caused by clinical chronic renal failure.(3)Total urine protein(UTP)at 24h: Compared with the empty control group,there was no significant difference in 24 h urine volume,urine volume and 24 h total urine were reduced to varying degrees,and the tubular atrophy or expansion was improved to varying degrees.The epidermal cells were relatively intact,and the inflammatory cells were relatively small.The tissues were significantly reduced,and the purple-yellow particles were significantly reduced in the glomeruli and glomeruli.(4)Plasma ELISA: Compared with the blank control group,the MDA in the plasma of the model group rats was statistically different(P<0.05),and the MDA levels in the plasma of the Gexiguna-3 soup and the positive control group were statistically different.(P<0.05);the plasma SOD level in Gexiguna-3 decoction group was statistically different(P<0.05);the plasma SOD activity level in positive control rats was statistically significant(P<0.05).Compared with the model group,the plasma MDA in Gexiguna-3 soup group had very significant statistical difference(P<0.001);the plasma SOD in Gexiguna-3 soup group had statistical difference(P<0.05).Compared with the positive control group,the MDA levels in the Gexiguna-3 decoction group were significantly different(P<0.001).(5)Changes in kidney tissue: The kidneys of the blank control group are like beans,with regular shapes,tough texture,elasticity,smooth surface,complete kidney structure,clear boundary between cortex and medulla,and complete glomerular and tubular structure.There was no significant change in the composition and quantity of cells in the glomeruli.The kidney tissues of the model group all had unilateral,bilateral or trilateral incisions.The renal tissues along the incision have formed fibrosis lesions(sclerosis)of varying sizes,and some fibrosis The lesions extend into the renal cortex and medulla,resulting in the formation of fibrosis areas of varying sizes within the renal cortex and medulla.The small fibrosis areas are in the form of thin cords or nodules,and the large fibrosis lesions are in irregular patches.The atrophy of the glomeruli and renal tubules in fibrotic lesions is different.More and less brown lipofuscin particles can be seen in the cytoplasm of atrophic tubular epithelial cells.Occasionally,some mice see scattered necrosis of tubular epithelial cells;renal tubules As the volume increases,compensatory hypertrophy occurs in varying degrees.The renal capsule around the incision was obviously thickened,and the surface was seen with villi-like connective tissue(organized cellulose),and some of the kidneys' local surface could see adhesions to the spleen and pancreas.Compared with the model group,the lesions of the Gexiguna-3 decoction group and the positive control group were reduced to varyingdegrees,and the tubular atrophy or expansion was improved to varying degrees.The epidermal cells were relatively intact,and the inflammatory cells were relatively small.The tissues were significantly reduced,and the purple-yellow particles were significantly reduced in the glomeruli and glomeruli.Conclusion Gexiguna-3 decoction can significantly improve the renal function(BUN,Scr,UA)of 5/6 nephrectomy-induced chronic renal failure rats,and delay the progress of chronic renal failure.Gexiguna-3 decoction can significantly reduce the 24 h urinary protein quantification(UTP)level in rats with chronic renal failure.Gexiguna-3 decoction can improve the general condition of chronic renal failure rats,delay the progression of chronic renal failure,the mechanism of action may be related to increasing SOD levels,reducing MDA expression,improving renal oxidative stress,and improving the quality of life of patients,delay the effect of entering dialysis time. |
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