Study On The Treatment Of Type ? Diabetes Mellitus With Soluble Microneedle-loaded Polypeptide Nanoparticles

Patients with type 2 diabetes(T2DM)have varying degrees of damage of islet ? and islet ? cell,and hence multiple drugs are often used clinically for combined treatment.In this study,insulin(INS)and glucagon-like peptides-exendin-4/(EX4)was selected to develop a new dosage which is capable of controlling the blood glucose while protecting the islet cells of T2 DM.In this study,we prepared the Nanoparticles(NPs)by an ionic gelation method,and the polymer microneedle was utilized as a drug carrier to achieve rapid and sustainable drug delivery.This study has remarkable innovation and considerable prospects,and it will probably replace traditional subcutaneous injection as a new model of T2 DM administration,eventually.In this study,DMNs comprised of carboxymethylcellulose sodium(CMC-Na),hyaluronic acid(HA),polyvinyl pyrrolidone(PVP),polyvinyl alcohol(PVA)with different material ratio combinations were prepared using the vacuum deposition.The texture analyzer and finite element analysis were utilized to test and simulate the mechanical properties of materials.CMC : PVP ratio of 2 : 1(15%(w / w))was utilized for needle preparation,and CMC : PVP ratio of 1 : 5(10%(w / w))was utilized for patches preparation.We prepared the NPs by an ionic gelation method,and dynamic high-pressure microfluidization(DHPM)was utilized to post-treat the NPs so as to improve the uniformity,repeatability,and controllability.The prepared NPs were spherical in appearance,uniform in size(119.1 nm),with good security and high stability.The entrapment efficiency(EE)of NPs was 85.89%,and the drug loading(LC)of NPs was 33.46%.This NPs were capable of completely releasing within 72 hours,and the therapeutic effect on T2 DM was also finer than traditional subcutaneous injection.Combining microneedle technology and nanotechnology,this study successfully prepared a sustained release,high-drug loading dry powder microneedle with good solubility,quick effect,and small skin damage.The finite element simulation was used to calculate the maximum flexion force of the microneedles and the skin von Mises stress.All of this will provide the necessary guidance for further microneedle transdermal administration.The DHPM was used to address most of the ongoing critical challenges such as longtime consumption,low yield,low repeatability,and easy contamination in amplification reactions during the manufacturing processes of the NPs.