Discussion On The Clinical Value Of HR-HPV E6/E7 MRNA Detection In Screening Of Cervical Precancerous Lesions

Objective: In many countries,the HR-HPV test method has been recommended as a preliminary screening method for cervical cancer,but studies have shown that the specificity of the HR-HPV DNA test method is low.As a newer cervical cancer screening technology,whether the HR-HPV E6/E7 mRNA detection method has higher clinical application value and greater advantages in accurate cervical cancer screening remains to be studied.The purpose of this study was to investigate the clinical application value of HR-HPV E6/E7 mRNA detection method in the screening of cervical precancerous lesions.Method: In this retrospective analysis,we enrolled 940 patients who the visited Second Hospital of Hebei Medical University between January 1,2017 and August 31,2018 due to thin-layer fluid-based cytology abnormalities,positive HR-HPV testing result.All 940 patients underwent thin-layer liquid-based cytology,transcolposcopy biopsy,and histopathological examination.Among them.In group A,total of 397 patients from January 1,2017 to October 10,2017 adopted our HR-HPV DNA test method,and in group B,a total of 543 patients from November 1,2017 to August 31,2018 adopted our HR-HPV E6/E7 mRNA detection method.Statistical analysis of the test results,with P <0.05 as the difference was statistically significant.Result: Taking the histological diagnosis as the "gold standard".In group A,the sensitivity of the HR-HPV DNA group was 90.91%,the specificity was 21.13%,and the accuracy rate was 44.33% in the screening histological results ? HSIL;The sensitivity of the TCT group was 77.27%,the specificity was 33.96%,and the accuracy rate was 48.36% in the screening histological results ? HSIL.Compared with the TCT group,the sensitivity of the HR-HPV DNA group were higher,and the specificity were lower.All the differences were statistically significant(P<0.05).The accuracy rate were lower,and the difference were not statistically significant(P>0.05).In group B,the HR-HPV E6/E7 mRNA group had a sensitivity of 88.37%,a specificity of 28.35%,and a accuracy rate of 52.12% in the screening histological results ? HSIL;The TCT group had a sensitivity of 74.88%,a specificity of 34.76%,and a accuracy rate of 50.64% in the screening histological results ? HSIL.Compared with the TCT group,the sensitivity of the HR-HPV E6/E7 mRNA group were higher,and the specificity were lower.All the differences were statistically significant(P<0.05).The accuracy rate were higher,and the difference were not statistically significant(P>0.05).Conclusions:1.Among the screening histological results?HSIL,the HR-HPV E6 / E7 mRNA detection method has the highest accuracy rate.Its sensitivity is higher than that of the TCT detection method,and its specificity is higher than that of the HR-HPV DNA detection method.2.Thus,The HR-HPV E6/E7 mRNA detection method has both excellent sensitivity and specificity.It has higher clinical application value in screening ?HSIL,which can improve the screening efficiency of cervical cancer,and can provide better options to achieve accurate cervical cancer screening.