The Study On The Correlation Between Non-coding RNA And Papillary Thyroid Microcarcinoma

Objective:To analyze the expression of circ RNA and lnc RNA in papillary thyroid microcarcinoma and the normal adjacent tissues.The differentially expressed circ RNA and lnc RNA were selected to lay the foundation for the subsequent research of biomarkers,and predict the mi RNA that interacts with circ RNA and lnc RNA,the construction of circ RNA/mi RNA/m RNA?lnc RNA/mi RNA/m RNA molecule regulatory network maps will provide new ideas for the possible occurrence?development?infiltration and invasion mechanism of papillary thyroid microcarcinoma.Methods:Five patients with pathological diagnosis of papillary thyroid microcarcinoma were selected in this study.The high-throughput sequencing method was used to detect circ RNA and lnc RNA in cancer tissue and normal adjacent tissue.GO enrichment analysis was to analyze the function of differential genes.KEGG pathway enrichment analysis was to find the differentially express signaling pathways.The Target Scan&mi Randa was used to predict the mi RNA that adsorbed by circ RNA and lnc RNA.The cotyscape was used to draw the circ RNA/mi RNA/m RNA,lnc RNA/mi RNA/m RNAmolecular regulatoynetwork maps.Results:1. The expression of circ RNA and lnc RNA in papillary thyroid microcarcinoma tissue are significantly different from that of adjacent normal adjacent tissues.circ RNA and lnc RNA can distinguish papillary thyroid microcarcinoma tissue from normal adjacent tissue well.2. There were 589 circ RNAs with statistically significant differ-ences in papillary thyroid microcarcinoma and normal adjacent tissues that were more than 2-fold,of which 354 were up-regulated and 235 were down-regulated.The circ RNAs with the most obvio usdifference between up-regulation and down-regulation were hsa-cir c-0050581 and has-circ-0072107.There are 402 lnc RNAs with statisti c-ally significant differences of more than 2-fold between the two groups,of which 103 are up-regulated and 299 are down-regulated.The most significant differences between up-regulation and down-regulation are NONHSAT162365.1 and NONHSAT181139.1.3.GO enrichment analysis results?1?GO enrichment results of the hostgene of differentially expressed circ RNA:The two groups of differential genes are concentrated in the combination of growth factors of molecular functions,and are mainly concentrated in participating in the formation of extracellular structures and extracellular matrix tissues for the biological processes.it is mainly concentrated on collagen trimer and basement membrane of cell composition.?2?GO enrichment results of the hostgene of differentially expressed lnc RNA:the cics GO enrichment analysis results of lnc RNA are that in molecular functions,the two groups of differential genes are mainly concentrated in the formation of protein complex scaffold.In metabolic processes the two groups of differential genes are mainly concentrated in the formation of the metabolic processes involved in phenol-containing compounds.In cell composition the two groups of differential genes are mainly concentrated in the polycystic body.The results of lnc RNA trans GO enrichment analysis are that in molecular function,the two groups of differential genes are concentrated in the combination of single-stranded RNA molecules,in biological processes the two groups of differential genes are mainly concentrated in the positive regulation of autophagy,in cell composition,the two groups of differential genes are mainly concentrated in axon cytoplasm.3.The results of KEEG pathway enrichment analysis showed that there were 247 signal pathways in cancer tissues and normal adjacent tissues,and there were 13 signal pathways with statistical differences.In the TGF-?signaling pathway,the expressions of TGF-?R1,TGF-?2,TGF-?3,THBS1,and INHBA were significantly up-regulated.TGF-?2was selected for the subsequent prediction study of mi RNA adsorption by circ RNA and lnc RNA.4.There are 32 mi RNA acting on TGF-?₂ in the TGF-?signal pathway,they are has-mi R-301a-3p?has-mi R-301b-3p?has-mi R-3064-5p?has-mi R-361-5p and so on,and the number of circ RNA which act as the sponge of mi RNA is 77.Among them has-circ-0027663 shows the most signification difference with 5.53 times.There are 10 lnc RNAs acting on this signal pathway,among them NOZHSAT131988.2 shows the most signification difference with 2.76times.Conclusion:1.Compared with normal thyroid tissue,the expression of circ RNA and lnc RNA in the tissue of papillary thyroid microcarcinoma was significantly deregulated.2.In papillary thyroid microcarcinoma tissue,hsa-circ-0050581and NONHSAT162365.1 were most up-regulated with 9.38 and 50.72 times respectively.3. As the sponge molecule of hsa-mi R5195-3p,has-circ-0027663can promote the occurrence of papillary thyroid microcarcinoma through the has-circ-0027663/has-mi R-5195-3p/TGF-?₂pathway.4. As the sponge molecule of has-mi R-6504-5p and has-mi R-142-3p.1,NOZHSAT131988.2 may promote the occurrence of thyroid micropapillary carcinoma through NOZHSAT131988.2/has-mi R-6504-5p/TGF-?2 and NOZHSAT131988.2/has-mi R-142-3p.1/TGF-?2 path-ways.