CircMYO10 Promotes Osteosarcoma Progression Via MiR-370-3p/RUVBL1 Axis

Background:CircMYO10 is a circular RNA generated by back-splicing of MYO10.CircMYO10 is upregulated in osteosarcoma cell lines,but its functional role in osteosarcoma is still unknown.This study aimed to clarify the mechanism of circMYO10 in osteosarcoma.Methods:CircMYO10 expression in 10 paired osteosarcoma and chondroma tissues was assessed by quantitative reverse transcription polymerase chain reaction(q PCR).The function of circMYO10/miR-370-3p/RUVBL1 axis was assessed regarding two key characteristics: proliferation and endothelial–mesenchymal transition(EMT).Bioinformatics analysis,western blotting,real-time PCR,RNA binding protein immunoprecipitation,dual-luciferase reporter assays,and rescue experiments were used to evaluate the mechanism.Stably transfected MG63 cells were injected via tail vein or subcutaneously into nude mice to assess the role of circMYO10 in vivo.Results:CircMYO10 was significantly upregulated,while miR-370-3p was downregulated,in osteosarcoma cell lines and human osteosarcoma samples.Either silencing circMYO10 or overexpressing miR-370-3p inhibited cell proliferation and EMT both in vivo and in vitro.Experiments showed that circMYO10 acts as a sponge for miR-370-3p to upregulate RUVBL1 expression.Either miR-370-3p inhibition or RUVBL1 overexpression abrogated the inhibition of proliferation,EMT of osteosarcoma cells in vitro and in vivo seen upon circMYO10 suppression? Conclusions:CircMYO10 promotes osteosarcoma progression by acting as a sponge for miR-370-3p to upregulateRUVBL1 expression.CircMYO10 may be a potential therapeutic target for osteosarcoma treatment.