| PART ? EFFECTS OF ADVANCED MATERNAL AGE ON GROWTH AND DEVELOPMENT OF OFFSPRING RATSObjective:To explore the effects of advanced maternal age on growth and development of offspring ratsMethods:A total of 10 3-month-old Sprague-Dawley rat femals and 1012-month-old ones were individually housed with randomly selected 3-month-old males(1:1),whose offspring were divided into Ctl group and AMA group.Recording the conception time,the number of births,sex percentage,death,body weight,eye opening process of the pups,and observe the developmental malformation of the offspring during one pregnancyResults:(1)Compared with Ctl group,the conception time of the AMA group is significantly longer(30.63±1.558 vs 23.80±0.6464,t=4.370,P=0.0005);(2)During one pregnancy,there was no significant difference in the litter size between the AMA and Ctl group(11.55±0.9664 vs 11.55±1.337,t=0,P=1).(3)Compared with the Ctl group,there was no significant difference in the sex of offspring.(5.909+0.8029 vs 4.636±0.7419,t=1.164,P=0.2580).(4)Compared with the Ctl group,there was not much difference in the death number of offspring(0.5455±0.3659 vs 0.3636±0.1521,t=0.4588,P=0.6513).(5)Compared with the Ctl group,the AMA group had a significant increase in the body weight within the first 2 weeks after delivery(P<0.05).(6)Compared with the Ctl group,the AMA group had a significant delay in eye opening process(1.118±0.2369 vs 1.788±0.06908,t=3.601,P=0.0007;1.147±0.2339 vs 1.988±0.0125,t=5.535,P<0.0001,respectively).(7)In the AMA group,we observed one case of limb deficiency,and another case of developmental deformity of the rat brain.Conclusion:Advanced maternal age can have a negative effect on growth and development of offspring rats,which is mainly manifested in the longer conception time,delayed eye opening process and weight gain of the offspring rats.At the same time,the risk of developing malformations in the AMA group may also increasePART ? EFFECTS OF ADVANCED MATERNAL AGE ON HIPPOCAMPAL NEURAL STEM CELL DEVELOPMENT IN OFFSPRING RATSObjective:To explore the effects and potential molecular mechanism of advanced maternal age on hippocampal neural stem cell development in offspring ratsMethods:A total of 10 3-month-old Sprague-Dawley rat femals and 1012-month-old ones were individually housed with randomly selected 3-month-old males(1:1,n=20),whose offspring were divided into Ctl group and AMA group.A total of 40 rats were randomly selected from each group,and hippocampus tissue samples were collected on day 7,14,28.Western blot and immunofluorescence technique were used to measure the protein expression of Nestin?Dcx?PSA-NCAM?NeuN and GFAP in the hippocampus tissueResults:(1)Compared with the control group,on day 7,the AMA group had significantly lower protein expression of Dcx(0.79±0.09 vs 1.32±0.19,t=2.457,P=0.0276),especially in hippocampus DG region(31.52±1.36 vs 37.74±0.91,t=3.806,P=0.0019);(2)The total protein expression of Nestin was not much different between the two groups on day 7(0.70±0.09 vs 0.87±0.15,t=0.9672,P=0.3498),but compared with the control group,the AMA group had significantly lower protein expression of Nestin in hippocampus DG region(25.67±2.46 vs 34.89±2.59,t=2.581,P=0.0218);(3)Compared with the control group,on day 14,the AMA group had significantly lower protein expression of PS A-NCAM in hippocampus DG region(26.36±1.31vs 33.42±1.52,t=3.523,P=0.0034);(4)Compared with the control group,on day 28,the AMA group had significantly lower protein expression of GFAP in hippocampus CA1?CA3 and DG region(24.27±0.98 vs 27.71±0.85,t=2.643,P=0.0193;26.14±1.14 vs 29.54±0.79,t=2.446,P=0.0282;25.21±0.87 vs 28.82±0.84,t=2.985,P=0.0098,respectively).But the protein expression of NeuN was not much different between the two groupsConclusion:Advanced maternal age may have a negative effect on hippocampal neural stem cell development in offspring rats by inhibiting the development,survival,migration and differentiation of hippocampal neural stem cells in the offspring. |
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