| Cerebral infarction, as a common disease of the central nervous system, seriously induces the coloboma of neural function. The emphasis of research cerebral infarction is how to effectively improve the recovery of neural function on the convalescence stage. Traditionary theory believes that the adult central nerve system couldn't recovery after injury. But the neural stem cells overturned the opinions above and brought new therapy on cerebral infarction. Considerable researchs had confirm that cerebral infarction can active endogenous neural stem cells and induce neurogenesis. The neurogenesis of cerebral infarction includes the proliferation, migration and differentiation of neural stem cells. After proliferation, the endogenous neural stem cells migrate to the injury regions and differentiate to the neural cells which can repair part of the neural functions. But there are few endogenous neural stem cells in the adult central neural system and many factors influence the course of neurogenesis. At present, how to activate endogenous neural stem cells and promote them to differentiate to neurons is the focus of research and the key point of using neural stem cells to treat cerebral infarction.Hippocampus, the key position of learning and memory, is often vulnerable to cerebral infarction. Injury of hippocampus may induce long-lasting impairment of the ability of learning and memory which is hard to be repaired. But hippocampus is also one important adult neurogenic region. In our research, we detected the influence of Water maze training on the proliferation and differentiation of neural stem cells after hippocampus infraction induced by photochemical method.Water maze training is one part of the rehabilitative training. In previous researches, we have found the water maze training could increase the expression of nerve growth factor, improve the migration of neural stem cells, enchance the plasticity of brain and improve the function of learning and memory after hippocampus infraction. It has few been reported of the effects of water maze training on the proliferation and differentiation of neural stem cells after infraction.By establishing the model of photochemically induced hippocampus infraction, we observed the effects of water maze training on the proliferation and differentiation of neural stem cells in the dentate gyrus (DG). We investigated the relationship between water maze training and plasticity which may contribute theoretically to the clinical rehabilitation therapy of cerebral stroke. The experiment consists of two parts.PAPT 1 The effect of water maze training on the expression of BrdU and Nestin in the dentate gyrus of rats with hippocampal infarctionObjective To explore the effect of water maze training on the proliferation of neural stem cells in the DG of hippocampus infarcted rats. Methods One hundred and eight Sprague-Dawley rats were randomized into infarction+ training group (1, 7, 14, 21, 28 and 35 days), infarction group (1, 7, 14, 21, 28 and 35 days) and control group (1, 7, 14, 21, 28 and 35 days). Photochemistry initiation was used to induce hippocampal infarction in infarction+training group, infarction group. At 1 day after surgery, Morris water maze training was used for infarction+training group, free-movement without training was performed for infarction group. Double staining immunofluorescence was used to detect the co-expression of bromodeoxyuridine (BrdU) with Nestin in the DG at different time points. Results Few BrdU/Nestin double staining cells were observed in the DG of control rats. In the infarction+training and infarction groups for 7, 14, 21 and 28 days, the number of BrdU/Nestin double-stained cells increased in the DG on the contralateral side compared with the control group (P<0.01). BrdU/Nestin double-stained cells increased obviously after 7, 14, 21 and 28 days in the infarction+training group compared with the infarction group(P<0.01). In the infarction+training and infarction groups for 35 days, no significant difference in the number of BrdU/Nestin double-stained cells in the DG on the contralateral side was found between them and the control group (P>0.05). Conclusion Water maze training can accelerate the proliferation of neural stem cells and then promote the recovery of neural function.PAPT 2 The effect of water maze training on neural stem cell differentiation in the dentate gyrus of rats with hippocampal infarctionObjective To explore the effect of water maze training on the differentiation of neural stem cells in the dentate gyrus(DG) of left hippocampal infacted rats. Methods One hundred and sixty-two Sprague-Dawley rats were randomized into infarction+ training group (1, 7, 14, 21, 28, 35, 42, 49 and 60 days), infarction group(1, 7, 14, 21, 28, 35, 42, 49 and 60 days) and control group(1, 7, 14, 21, 28, 35, 42, 49 and 60 days). Photochemistry was used to induce hippocampal infarction in infarction+ training group, infarction group. At 1 day after surgery, Morris water maze training was used for infarction+training group, free-movement without training was performed for infarction group. Double staining immunofluorescence was used to detect the co-expression of bromodeoxyuridine (BrdU) with neuronal nuclei antigen (NeuN) or glia fibrillary acidic protein (GFAP) in the DG at different time points. Results Few BrdU/NeuN and BrdU/GFAP double staining cells were observed in the DG of control rats. In the infarction+training groups, at 14, 21, 28, 35, 42 and 49 day after surgery, the number of BrdU/NeuN and BrdU/GFAP double-stained cells increased in the DG on the contralateral side compared with the control group (P<0.01). Obviously more BrdU/NeuN and BrdU/GFAP double-stained cells were found in the infarction+ training group than that in the infarction group at 14, 21, 28, 35, 42 and 49 day(P<0.01). In the infarction+training and infarction groups for 60 days, no significant difference in the number of BrdU/NeuN and BrdU/GFAP double-stained cells in the DG on the contralateral side was found between them and the control group (P>0.05). Conclusion Water maze training can accelerate the capability of the differentiation of neural stem cells to neurons and astrocytes, by which to promote the recovery of neural functions. |
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